{"doi":"10.1091/mbc.e07-10-1069","title":"A Multicomponent Assembly Pathway Contributes to the Formation of Acentrosomal Microtubule Arrays in Interphase\n                    <i>Drosophila</i>\n                    Cells","abstract":"<jats:p>In animal cells, centrosomes nucleate microtubules that form polarized arrays to organize the cytoplasm. Drosophila presents an interesting paradox however, as centrosome-deficient mutant animals develop into viable adults. To understand this discrepancy, we analyzed behaviors of centrosomes and microtubules in Drosophila cells, in culture and in vivo, using a combination of live-cell imaging, electron microscopy, and RNAi. The canonical model of the cycle of centrosome function in animal cells states that centrosomes act as microtubule-organizing centers throughout the cell cycle. Unexpectedly, we found that many Drosophila cell-types display an altered cycle, in which functional centrosomes are only present during cell division. On mitotic exit, centrosomes disassemble producing interphase cells containing centrioles that lack microtubule-nucleating activity. Furthermore, steady-state interphase microtubule levels are not changed by codepleting both γ-tubulins. However, γ-tubulin RNAi delays microtubule regrowth after depolymerization, suggesting that it may function partially redundantly with another pathway. Therefore, we examined additional microtubule nucleating factors and found that Mini-spindles, CLIP-190, EB1, or dynein RNAi also delayed microtubule regrowth; surprisingly, this was not further prolonged when we codepleted γ-tubulins. Taken together, these results modify our view of the cycle of centrosome function and reveal a multi-component acentrosomal microtubule assembly pathway to establish interphase microtubule arrays in Drosophila.</jats:p>","journal":"Molecular Biology of the Cell","year":2008,"id":43184,"datarank":5.606420638003084,"base_score":4.948759890378168,"endowment":4.948759890378168,"self_citation_contribution":0.7423139835567254,"citation_network_contribution":4.864106654446359,"self_endowment_contribution":0.7423139835567254,"citer_contribution":4.864106654446359,"corpus_percentile":null,"corpus_rank":null,"citation_count":140,"citer_count":116,"citers_with_citation_signal":105,"citers_with_endowment":105,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":205637,"name":"Nasser M. Rusan","orcid":null,"position":1,"is_corresponding":false},{"id":203139,"name":"Mark Peifer","orcid":null,"position":2,"is_corresponding":false},{"id":205638,"name":"Stephen L. Rogers","orcid":null,"position":3,"is_corresponding":false},{"id":205636,"name":"Gregory C. Rogers","orcid":"0000-0003-1870-1713","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.948759890378168,"endowment":4.948759890378168,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18463166","pmcid":"PMC2441692","openalex_id":"https://openalex.org/W2168614708","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM067236","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01GM67236","title":null}],"total_grants":2,"fwci":4.8641,"citation_percentile":0.95713168,"influential_citations":7,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":10},{"year":2014,"count":9},{"year":2015,"count":8},{"year":2016,"count":6},{"year":2017,"count":7},{"year":2018,"count":10},{"year":2019,"count":9},{"year":2020,"count":7},{"year":2021,"count":12},{"year":2022,"count":8},{"year":2023,"count":5},{"year":2024,"count":3},{"year":2025,"count":2},{"year":2026,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2441692","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc2441692?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2441692","host_type":"repository"},{"url":"https://doi.org/10.1091/mbc.e07-10-1069","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18463166","host_type":"repository"}],"fields_of_study":["Microtubule and mitosis dynamics","Protist diversity and phylogeny","Plant Molecular Biology Research","Biology","Medicine","Animals","Centrioles","Centrosome","Drosophila melanogaster","Dyneins","Gene Expression Regulation","Green Fluorescent Proteins","Interphase","Microscopy, Electron, Transmission","Microscopy, Fluorescence","Mitosis","Models, Biological","RNA Interference","Spindle Apparatus","Tubulin"],"mesh_terms":["Animals","Centrioles","Drosophila melanogaster","Dyneins","Gene Expression Regulation","Interphase","Microscopy, Fluorescence","Mitosis","Spindle Apparatus","Models, Biological","Tubulin","Centrosome","RNA Interference","Microscopy, Electron, Transmission","Green Fluorescent Proteins"],"keywords":["Centrosome","Cell biology","Biology","Microtubule","Centriole","Centrosome cycle","Microtubule organizing center","Microtubule nucleation","Mitosis","Interphase","Astral microtubules","Cell division","Cell cycle","Genetics","Cell"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T19:48:50.811365Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}