{"doi":"10.1091/mbc.e06-07-0606","title":"Novel Function of Clathrin Light Chain in Promoting Endocytic Vesicle Formation","abstract":"<jats:p>Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts with members of the Hip1/R family of endocytic proteins, including the budding yeast homologue, Sla2p. Here, using in vivo analysis in yeast, we provide novel insight into the role of this interaction. We find that overexpression of LC partially restores endocytosis to cells lacking clathrin HC. This suppression is dependent on the Sla2p binding region of LC. Using live cell imaging techniques to visualize endocytic vesicle formation, we find that the N-terminal Sla2p binding region of LC promotes the progression of arrested Sla2p patches that form in the absence of HC. We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation.</jats:p>","journal":"Molecular Biology of the Cell","year":2006,"id":24641,"datarank":2.9219450020749425,"base_score":3.8501476017100584,"endowment":3.8501476017100584,"self_citation_contribution":0.5775221402565088,"citation_network_contribution":2.3444228618184337,"self_endowment_contribution":0.5775221402565088,"citer_contribution":2.3444228618184337,"corpus_percentile":null,"corpus_rank":null,"citation_count":46,"citer_count":43,"citers_with_citation_signal":35,"citers_with_endowment":35,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":147278,"name":"Fatima-Zahra Idrissi","orcid":null,"position":1,"is_corresponding":false},{"id":147279,"name":"Maria Isabel Geli","orcid":null,"position":2,"is_corresponding":false},{"id":147280,"name":"Sandra K. Lemmon","orcid":null,"position":3,"is_corresponding":false},{"id":147277,"name":"Thomas M. Newpher","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.8501476017100584,"endowment":3.8501476017100584,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16870700","pmcid":"PMC1635359","openalex_id":"https://openalex.org/W2134098725","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM055796","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM55796","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM08056-20","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM008056","title":null}],"total_grants":4,"fwci":1.7766,"citation_percentile":0.8366063,"influential_citations":3,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":4},{"year":2014,"count":2},{"year":2016,"count":5},{"year":2018,"count":1},{"year":2019,"count":3},{"year":2020,"count":1},{"year":2022,"count":2},{"year":2023,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"http://doi.org/10.1091/mbc.E06-07-0606","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc1635359?pdf=render","host_type":"GREEN"},{"url":"http://doi.org/10.1091/mbc.E06-07-0606","host_type":"repository"},{"url":"https://doi.org/10.1091/mbc.e06-07-0606","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16870700","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Toxin Mechanisms and Immunotoxins","Plant Reproductive Biology","Biology","Medicine","Binding Sites","Carrier Proteins","Clathrin Heavy Chains","Clathrin Light Chains","Clathrin-Coated Vesicles","Cytoskeletal Proteins","Models, Biological","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Transport Vesicles"],"mesh_terms":["Binding Sites","Carrier Proteins","Cytoskeletal Proteins","Models, Biological","Saccharomyces cerevisiae","Transport Vesicles","Clathrin-Coated Vesicles","Saccharomyces cerevisiae Proteins","Clathrin Heavy Chains","Clathrin Light Chains"],"keywords":["Clathrin","Endocytic cycle","Endocytosis","Clathrin adaptor proteins","Biology","Cell biology","Vesicle","Signal transducing adaptor protein","Receptor-mediated endocytosis","Biochemistry","Cell","Membrane","Signal transduction"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T22:54:01.727933Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}