{"doi":"10.1091/mbc.e04-09-0771","title":"Mammalian Protein SCP1 Forms Synaptonemal Complex-like Structures in the Absence of Meiotic Chromosomes","abstract":"<jats:p>Synaptonemal complexes (SCs) are evolutionary conserved, meiosis-specific structures that play a central role in synapsis of homologous chromosomes, chiasmata distribution, and chromosome segregation. However, it is still for the most part unclear how SCs do assemble during meiotic prophase. Major components of mammalian SCs are the meiosis-specific proteins SCP1, 2, and 3. To investigate the role of SCP1 in SC assembly, we expressed SCP1 in a heterologous system, i.e., in COS-7 cells that normally do not express SC proteins. Notably, under these experimental conditions SCP1 is able to form structures that closely resemble SCs (i.e., polycomplexes). Moreover, we show that mutations that modify the length of the central α-helical domain of SCP1 influence the width of polycomplexes. Finally, we demonstrate that deletions of the nonhelical N- or C-termini both affect polycomplex assembly, although in a different manner. We conclude that SCP1 is a primary determinant of SC assembly that plays a key role in synapsis of homologous chromosomes.</jats:p>","journal":"Molecular Biology of the Cell","year":2005,"id":615626,"datarank":0.7168685239667295,"base_score":4.77912349311153,"endowment":4.77912349311153,"self_citation_contribution":0.7168685239667295,"citation_network_contribution":0.0,"self_endowment_contribution":0.7168685239667295,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":118,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1586904,"name":"Manfred Alsheimer","orcid":null,"position":1,"is_corresponding":false},{"id":1510056,"name":"Ricardo Benavente","orcid":null,"position":2,"is_corresponding":false},{"id":63909,"name":"Rupert Öllinger","orcid":"0000-0002-2292-5982","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Mammalian Protein SCP1 Forms Synaptonemal Complex-like Structures in the Absence of Meiotic Chromosomes","abstract":"<jats:p>Synaptonemal complexes (SCs) are evolutionary conserved, meiosis-specific structures that play a central role in synapsis of homologous chromosomes, chiasmata distribution, and chromosome segregation. However, it is still for the most part unclear how SCs do assemble during meiotic prophase. Major components of mammalian SCs are the meiosis-specific proteins SCP1, 2, and 3. To investigate the role of SCP1 in SC assembly, we expressed SCP1 in a heterologous system, i.e., in COS-7 cells that normally do not express SC proteins. Notably, under these experimental conditions SCP1 is able to form structures that closely resemble SCs (i.e., polycomplexes). Moreover, we show that mutations that modify the length of the central α-helical domain of SCP1 influence the width of polycomplexes. Finally, we demonstrate that deletions of the nonhelical N- or C-termini both affect polycomplex assembly, although in a different manner. We conclude that SCP1 is a primary determinant of SC assembly that plays a key role in synapsis of homologous chromosomes.</jats:p>","is_dataset_classified":null,"base_score":4.77912349311153,"endowment":4.77912349311153,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15496453","pmcid":"PMC539165","openalex_id":"https://openalex.org/W2099340636","authors":[],"funders":[],"total_grants":0,"fwci":2.8739,"citation_percentile":0.9093694,"influential_citations":0,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":2},{"year":2014,"count":5},{"year":2015,"count":6},{"year":2016,"count":6},{"year":2017,"count":4},{"year":2018,"count":5},{"year":2019,"count":10},{"year":2020,"count":8},{"year":2021,"count":8},{"year":2022,"count":3},{"year":2023,"count":5},{"year":2026,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/539165","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/539165","host_type":"repository"},{"url":"https://doi.org/10.1091/mbc.e04-09-0771","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15496453","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC539165","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","Mitochondrial Function and Pathology","Microtubule and mitosis dynamics","Animals","COS Cells","Chromosome Pairing","Chromosomes","Cytoplasm","DNA, Complementary","DNA-Binding Proteins","Male","Meiosis","Microscopy, Electron","Microscopy, Fluorescence","Mutation","Nuclear Proteins","Protein Structure, Tertiary","Rats","Rats, Wistar","Spermatocytes","Synaptonemal Complex","Transfection"],"mesh_terms":["Animals","Chromosomes","Cytoplasm","DNA-Binding Proteins","Male","Meiosis","Microscopy, Electron","Microscopy, Fluorescence","Mutation","Nuclear Proteins","Spermatocytes","Synaptonemal Complex","Transfection","Rats, Wistar","Protein Structure, Tertiary","DNA, Complementary","COS Cells","Chromosome Pairing","Rats"],"keywords":["Synapsis","Synaptonemal complex","Biology","Homologous chromosome","Meiosis","Chiasma","Genetics","Prophase","Bivalent (engine)","Cell biology","Chromosomal crossover","Chromosome segregation","Heterologous","Chromosome","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T20:36:25.144402Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}