{"doi":"10.1091/mbc.e02-09-0558","title":"Direct Evidence for a Critical Role of Myosin II in Budding Yeast Cytokinesis and the Evolvability of New Cytokinetic Mechanisms in the Absence of Myosin II","abstract":"<jats:p>In the budding yeast Saccharomyces cerevisiae, an actomyosin-based contractile ring is present during cytokinesis, as occurs in animal cells. However, the precise requirement for this structure during budding yeast cytokinesis has been controversial. Here we show that deletion of MYO1, the single myosin II gene, is lethal in a commonly used strain background. The terminal phenotype of myo1Δ is interconnected chains of cells, suggestive of a cytokinesis defect. To further investigate the role of Myo1p in cytokinesis, we conditionally disrupted Myo1 function by using either a dominant negative Myo1p construct or a strain where expression of Myo1p can be shut-off. Both ways of disruption of Myo1 function result in a failure in cytokinesis. Additionally, we show that amyo1Δ strain previously reported to grow nearly as well as the wild type contains a single genetic suppressor that alleviates the severe cytokinesis defects of myo1Δ. Using fluorescence time-lapse imaging and electron microscopy techniques, we show that cytokinesis in this strain is achieved through formation of multiple aberrant septa. Taken together, these results strongly suggest that the actomyosin ring is crucial for successful cytokinesis in budding yeast, but new cytokinetic mechanisms can evolve through genetic changes when myosin II function is impaired.</jats:p>","journal":"Molecular Biology of the Cell","year":2003,"id":42078,"datarank":3.6928585760258343,"base_score":4.442651256490317,"endowment":4.442651256490317,"self_citation_contribution":0.6663976884735476,"citation_network_contribution":3.0264608875522865,"self_endowment_contribution":0.6663976884735476,"citer_contribution":3.0264608875522865,"corpus_percentile":null,"corpus_rank":null,"citation_count":84,"citer_count":79,"citers_with_citation_signal":68,"citers_with_endowment":68,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201890,"name":"Maria Pitcher","orcid":null,"position":1,"is_corresponding":false},{"id":15011,"name":"Rong Li","orcid":"0000-0003-0587-8130","position":2,"is_corresponding":false},{"id":201889,"name":"Nicola Tolliday","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.442651256490317,"endowment":4.442651256490317,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12589071","pmcid":"PMC150009","openalex_id":"https://openalex.org/W2111209294","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM059964","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM59964","title":null}],"total_grants":2,"fwci":2.635,"citation_percentile":0.90067953,"influential_citations":10,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":4},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2016,"count":4},{"year":2017,"count":4},{"year":2018,"count":2},{"year":2019,"count":4},{"year":2020,"count":2},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/150009","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc150009?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/150009","host_type":"repository"},{"url":"https://doi.org/10.1091/mbc.e02-09-0558","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12589071","host_type":"repository"}],"fields_of_study":["Fungal and yeast genetics research","Genetic Neurodegenerative Diseases","Biofuel production and bioconversion","Biology","Medicine","Actomyosin","Cell Division","Genes, Dominant","Genetic Vectors","Genotype","Microscopy, Confocal","Microscopy, Electron","Microscopy, Fluorescence","Models, Biological","Myosin Type II","Phenotype","Plasmids","Saccharomyces cerevisiae","Saccharomycetales","Time Factors"],"mesh_terms":["Actomyosin","Cell Division","Saccharomycetales","Genes, Dominant","Genetic Vectors","Genotype","Microscopy, Electron","Microscopy, Fluorescence","Models, Biological","Phenotype","Plasmids","Saccharomyces cerevisiae","Time Factors","Microscopy, Confocal","Myosin Type II"],"keywords":["Cytokinesis","Septin","Biology","Cell biology","Myosin","Saccharomyces cerevisiae","Budding","Cell division","Genetics","Yeast","Cell"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-13T19:39:55.301095Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}