{"doi":"10.1089/vim.2023.0153","title":"Characterization of Two Highly Specific Monoclonal Antibodies Targeting the Glycan Loop of the Zika Virus Envelope Protein","abstract":"Zika virus (ZIKV) is an emerging flavivirus associated with several neurological diseases such as Guillain-Barré syndrome in adults and microcephaly in newborn children. Its distribution and mode of transmission (via Aedes aegypti and Aedes albopictus mosquitoes) collectively cause ZIKV to be a serious concern for global health. High genetic homology of flaviviruses and shared ecology is a hurdle for accurate detection. Distinguishing infections caused by different viruses based on serological recognition can be misleading as many anti-flavivirus monoclonal antibodies (mAbs) discovered to date are highly cross-reactive, especially those against the envelope (E) protein. To provide more specific research tools, we produced ZIKV E directed hybridoma cell lines and characterized two highly ZIKV-specific mAb clones (mAbs A11 and A42) against several members of the Flavivirus genus. Epitope mapping of mAb A11 revealed glycan loop specificity in Domain I of the ZIKV E protein. The development of two highly specific mAbs targeting the surface fusion protein of ZIKV presents a significant advancement in research capabilities as these can be employed as essential tools to enhance our understanding of ZIKV identification on infected cells ex vivo or in culture.","journal":"Viral Immunology","year":2024,"id":476588,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.962,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":482236,"name":"Brien K. Haun","orcid":null,"position":1,"is_corresponding":false},{"id":744738,"name":"Albert To","orcid":"0000-0002-7118-9803","position":2,"is_corresponding":false},{"id":1314125,"name":"Ludwig I. Mayerlen","orcid":"0009-0002-4083-0802","position":3,"is_corresponding":false},{"id":244153,"name":"Liana Medina","orcid":null,"position":4,"is_corresponding":false},{"id":482237,"name":"Chih-Yun Lai","orcid":null,"position":5,"is_corresponding":false},{"id":481621,"name":"Teri Ann S. Wong","orcid":"0000-0002-7110-657X","position":6,"is_corresponding":false},{"id":822033,"name":"Eileen Nakano","orcid":null,"position":7,"is_corresponding":false},{"id":1314514,"name":"Daniel Strange","orcid":null,"position":8,"is_corresponding":false},{"id":1314515,"name":"Draven L. Aquino","orcid":null,"position":9,"is_corresponding":false},{"id":533118,"name":"Yan‐Jang S. Huang","orcid":"0000-0002-9687-9341","position":10,"is_corresponding":false},{"id":533119,"name":"Stephen Higgs","orcid":"0000-0003-2436-1030","position":11,"is_corresponding":false},{"id":533120,"name":"Dana L. Vanlandingham","orcid":"0000-0002-7436-5921","position":12,"is_corresponding":false},{"id":1314126,"name":"A Lopez Garcia","orcid":"0000-0002-3005-1509","position":13,"is_corresponding":false},{"id":1127300,"name":"John M. Berestecky","orcid":"0009-0007-4917-991X","position":14,"is_corresponding":false},{"id":481623,"name":"Axel T. Lehrer","orcid":"0000-0002-0518-203X","position":15,"is_corresponding":false},{"id":365905,"name":"Alex R. McLaury","orcid":null,"position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T02:06:29.605760Z","pmid":"38574259","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}