{"doi":"10.1089/scd.2019.0101","title":"Dynamic Changes of Mouse Embryonic Stem Cell-Derived Neural Stem Cells Under In Vitro Prolonged Culture and Hypoxic Conditions","abstract":null,"journal":"Stem Cells and Development","year":2019,"id":663381,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1731966,"name":"Vuong Cat Khanh","orcid":null,"position":1,"is_corresponding":false},{"id":1731967,"name":"Saori Shirota","orcid":null,"position":2,"is_corresponding":false},{"id":1731968,"name":"Toshiki Kato","orcid":null,"position":3,"is_corresponding":false},{"id":1731969,"name":"Mami Matsuo Takasaki","orcid":null,"position":4,"is_corresponding":false},{"id":1721694,"name":"Osamu Ohneda","orcid":null,"position":5,"is_corresponding":false},{"id":1731965,"name":"Donny Lukmanto","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dynamic Changes of Mouse Embryonic Stem Cell-Derived Neural Stem Cells Under In Vitro Prolonged Culture and Hypoxic Conditions","abstract":"Embryonic stem (ES) cells have been utilized as an excellent model for the study of neural development. However, the dynamic changes of ES cell-derived neural stem cells (ES-NSCs), under the effects of prolonged cell culture and hypoxic conditions, are still obscured. In the present study, using the combination of serum-free culture of embryoid body-like aggregates (SFEB) culture and cell sorting by Sox-1, the ES-NSCs were easily isolated and showed in vitro temporal neural specification, which resulted in distinct cell fates after neural differentiation. Early passaged ES-NSCs gave rise to neurons, whereas late-passaged ES-NSCs gave rise to glial cells, similar to the in vivo dynamic changes during the neural development. Remarkably, hypoxia treatment induced the neural differentiation of ES-NSCs but did not affect the cell fate. Under hypoxic conditions, early passaged ES-NSCs showed the upregulation of neuronal markers, whereas late-passaged ES-NSCs showed the upregulation of a glial marker. In addition, the knockdown of the hypoxia-inducible factor 1α expression impaired the neuronal differentiation of early passaged ES-NSCs under hypoxic conditions. These data demonstrated the distinct effects of prolonged culture and hypoxic stimuli on the neural differentiation of ES-NSCs; prolonged culture was involved in the cell fate after neural differentiation, while hypoxia treatment efficiently promoted neural differentiation.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31507235","pmcid":null,"openalex_id":"https://openalex.org/W2972837166","authors":[],"funders":[],"total_grants":0,"fwci":0.2277,"citation_percentile":0.51700765,"influential_citations":0,"citation_trend":[{"year":2020,"count":2},{"year":2021,"count":1},{"year":2024,"count":2}],"oa_status":"closed","license":"https://www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/","oa_locations":[{"url":"https://www.liebertpub.com/doi/full-xml/10.1089/scd.2019.0101","host_type":"publisher"},{"url":"https://www.liebertpub.com/doi/pdf/10.1089/scd.2019.0101","host_type":"publisher"},{"url":"https://doi.org/10.1089/scd.2019.0101","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31507235","host_type":"repository"}],"fields_of_study":["Pluripotent Stem Cells Research","Neurogenesis and neuroplasticity mechanisms","3D Printing in Biomedical Research","Animals","Cell Hypoxia","Cells, Cultured","Embryoid Bodies","Flow Cytometry","Hypoxia-Inducible Factor 1, alpha Subunit","Mice","Mouse Embryonic Stem Cells","Neural Stem Cells","Neurogenesis","Neuroglia","Neurons","RNA Interference","RNA, Small Interfering","SOXB1 Transcription Factors"],"mesh_terms":["Mouse Embryonic Stem Cells","Animals","Cells, Cultured","Flow Cytometry","Neuroglia","Neurons","Cell Hypoxia","RNA Interference","RNA, Small Interfering","Mice","Hypoxia-Inducible Factor 1, alpha Subunit","Neurogenesis","SOXB1 Transcription Factors","Embryoid Bodies","Neural Stem Cells"],"keywords":["Neural stem cell","Biology","Embryonic stem cell","Neural cell","Cell biology","Embryoid body","Neural development","Neurogenesis","Cellular differentiation","Stem cell","Cell sorting","Adult stem cell","Immunology","Cell","Flow cytometry","Genetics","Hypoxia","Neural differentiation","Cell Fate","Es Cell-derived Neural Stem Cells","Prolonged Cell Culture"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T21:31:28.994970Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}