{"doi":"10.1089/oli.1.1997.7.79","title":"A Helix 1-Extended Hairpin Ribozyme Exhibits Altered Cleavage Behavior\n                    <i>In Vitro</i>","abstract":null,"journal":"Antisense and Nucleic Acid Drug Development","year":1997,"id":674113,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1761297,"name":"MARTIN TABLER","orcid":null,"position":1,"is_corresponding":false},{"id":1761296,"name":"JUTTA MOOSBAUER","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Helix 1-Extended Hairpin Ribozyme Exhibits Altered Cleavage Behavior\n                    <i>In Vitro</i>","abstract":"The catalytic domain of a hairpin ribozyme was incorporated at the 3'-end of a 254-base antisense RNA directed against the RNA of human immunodeficiency virus type 1 (HIV-1), generating a hairpin ribozyme with a largely extended helix 1. In parallel, a catalytic antisense RNA based on a hammerhead ribozyme was directed toward the same cleavage motif in the HIV-1 target. Both ribozymes were expected to create identical cleavage products. Cleavage analysis in vitro confirmed that the hammerhead ribozyme delivered the expected cleavage products. However, the helix 1-extended hairpin ribozyme catalyzed additional RNA cleavage at several unexpected sites, which were mapped. Some of the 3' cleavage products had other nucleotides than G at their 5'-terminus, indicating that the helix 1-extended hairpin ribozyme was able to cleave bonds other than NpG+1. Inspection of the sequence context of the different cleavage sites suggested that unconventional helices 2 in combination with an asymmetric loop A consisting of up to 32 unpaired nucleotides in the substrate strand were formed. A second variant of a helix 1-extended hairpin ribozyme that differed in two nucleotides gave consistent results.","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9149843","pmcid":null,"openalex_id":"https://openalex.org/W2323268835","authors":[],"funders":[],"total_grants":0,"fwci":0.473,"citation_percentile":0.63330106,"influential_citations":0,"citation_trend":[{"year":2022,"count":1}],"oa_status":"closed","license":"http://www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/","oa_locations":[{"url":"http://www.liebertpub.com/doi/pdf/10.1089/oli.1.1997.7.79","host_type":"publisher"},{"url":"https://doi.org/10.1089/oli.1.1997.7.79","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9149843","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","Advanced biosensing and bioanalysis techniques","Bacteriophages and microbial interactions","HIV-1","In Vitro Techniques","Nucleic Acid Conformation","Plasmids","RNA, Antisense","RNA, Catalytic","RNA, Viral","Restriction Mapping","Sequence Analysis, RNA","Substrate Specificity","Transcription, Genetic"],"mesh_terms":["Nucleic Acid Conformation","Plasmids","RNA, Viral","Substrate Specificity","Transcription, Genetic","Restriction Mapping","HIV-1","RNA, Catalytic","RNA, Antisense","Sequence Analysis, RNA","In Vitro Techniques"],"keywords":["Hairpin ribozyme","Ribozyme","VS ribozyme","Mammalian CPEB3 ribozyme","Ligase ribozyme","Hammerhead ribozyme","Stem-loop","Cleavage (geology)","RNA","Nucleotide","Base pair","Biology","Stereochemistry","Chemistry","Biochemistry","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T15:38:51.307622Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}