{"doi":"10.1089/jop.2005.21.429","title":"Stretch-Stimulated Activity of Large Conductance Calcium-Activated Potassium Channels in Human Retinal Pigment Epithelial Cells","abstract":null,"journal":"Journal of Ocular Pharmacology and Therapeutics","year":2005,"id":658210,"datarank":0.879256696910512,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.4633683885745447,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.4633683885745447,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":12,"citers_with_citation_signal":11,"citers_with_endowment":11,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1718236,"name":"Sheng-Nan Wu","orcid":null,"position":1,"is_corresponding":false},{"id":1718237,"name":"Dan-Ning Hu","orcid":null,"position":2,"is_corresponding":false},{"id":1718234,"name":"Shwu-Jiuan Sheu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Stretch-Stimulated Activity of Large Conductance Calcium-Activated Potassium Channels in Human Retinal Pigment Epithelial Cells","abstract":"AIM: The aim of this study was to investigate the effect of membrane stretch on the activity of BK(Ca) channels and determine whether change in BK(Ca)-channel activity induced by membrane stretch can be altered in the presence of riluzole. METHODS: The effect and mechanisms of membrane stretch on the activity of large-conductance Ca(2+)-activated K(+) (BK(Ca)) channels in human retinal pigment epithelial (RPE) cells were investigated using the patch-clamp technique. RESULTS: In cell-attached patches, an increase in BK(Ca)-channel activity was induced by membrane stretch, which is mainly mediated by the increase in the number of long-lived openings. With inside-out patches, application of pipette suction did not modify single-channel conductance of BK(Ca) channels; however, it did enhance the channel activity. Membrane stretch-induced channel activity was found to be independent of the level of intracellular Ca(2+). Membrane stretch also potentiated riluzole-stimulated BK(Ca)-channel activity. CONCLUSIONS: BK(Ca) channel can be stimulated by membrane stretch in human RPE cells. During an increase in tensile strength of RPE, the BK(Ca) channel might exert a protective action against deleterious tension by promoting a hyperpolarizing outflow that forces the cell to rest.","is_dataset_classified":null,"base_score":2.772588722239781,"endowment":2.772588722239781,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16386084","pmcid":null,"openalex_id":"https://openalex.org/W2088382683","authors":[],"funders":[],"total_grants":0,"fwci":0.4749,"citation_percentile":0.61682047,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"http://www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/","oa_locations":[{"url":"http://www.liebertpub.com/doi/pdf/10.1089/jop.2005.21.429","host_type":"publisher"},{"url":"https://doi.org/10.1089/jop.2005.21.429","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16386084","host_type":"repository"}],"fields_of_study":["Ion channel regulation and function","Ion Channels and Receptors","Neuroscience and Neural Engineering","Cell Line","Cell Membrane","Epithelial Cells","Excitatory Amino Acid Antagonists","Humans","Large-Conductance Calcium-Activated Potassium Channels","Patch-Clamp Techniques","Pigment Epithelium of Eye","Riluzole","Tensile Strength"],"mesh_terms":["Cell Line","Cell Membrane","Epithelial Cells","Humans","Pigment Epithelium of Eye","Tensile Strength","Patch-Clamp Techniques","Excitatory Amino Acid Antagonists","Riluzole","Large-Conductance Calcium-Activated Potassium Channels"],"keywords":["BK channel","Biophysics","Chemistry","Potassium channel","Pipette","Patch clamp","Calcium-activated potassium channel","Membrane potential","Cell biology","Biochemistry","Biology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T03:36:20.753583Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}