{"doi":"10.1089/hum.2021.283","title":"Strategies for Optimization of the Clustered Regularly Interspaced Short Palindromic Repeat-Based Genome Editing System for Enhanced Editing Specificity","abstract":"<jats:p>The clustered regularly interspaced short palindromic repeats (CRISPR) system is inarguably the most valuable gene editing tool ever discovered. Currently, three classes of CRISPR-based genome editing systems have been developed for gene editing, including CRISPR/CRISPR associate system (Cas) nucleases, base editors, and prime editors. Ever-evolving CRISPR technology plays an important role in medicine; however, the biggest obstacle to its use in clinical practice is the induction of off-target effects (OTEs) during targeted editing. Therefore, continuous improvement and optimization of the CRISPR system for reduction of OTEs is a major focus in the field of CRISPR research. This review aims to provide a comprehensive guide for optimization of the CRISPR-based genome editing system.</jats:p>","journal":"Human Gene Therapy","year":2022,"id":589751,"datarank":0.1784681992143775,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.01367635591416106,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.01367635591416106,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1508900,"name":"Hao-zheng Wang","orcid":null,"position":1,"is_corresponding":false},{"id":1508901,"name":"Ying-zhen Jian","orcid":null,"position":2,"is_corresponding":false},{"id":1508902,"name":"Zhong-tao Luo","orcid":null,"position":3,"is_corresponding":false},{"id":1508903,"name":"Hong-wei Shao","orcid":null,"position":4,"is_corresponding":false},{"id":636312,"name":"Wenfeng Zhang","orcid":"0000-0003-0784-5228","position":5,"is_corresponding":false},{"id":1508899,"name":"Yang-min Wang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Strategies for Optimization of the Clustered Regularly Interspaced Short Palindromic Repeat-Based Genome Editing System for Enhanced Editing Specificity","abstract":"<jats:p>The clustered regularly interspaced short palindromic repeats (CRISPR) system is inarguably the most valuable gene editing tool ever discovered. Currently, three classes of CRISPR-based genome editing systems have been developed for gene editing, including CRISPR/CRISPR associate system (Cas) nucleases, base editors, and prime editors. Ever-evolving CRISPR technology plays an important role in medicine; however, the biggest obstacle to its use in clinical practice is the induction of off-target effects (OTEs) during targeted editing. Therefore, continuous improvement and optimization of the CRISPR system for reduction of OTEs is a major focus in the field of CRISPR research. This review aims to provide a comprehensive guide for optimization of the CRISPR-based genome editing system.</jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34963339","pmcid":null,"openalex_id":"https://openalex.org/W4200005163","authors":[],"funders":[],"total_grants":0,"fwci":0.1481,"citation_percentile":0.46078591,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2024,"count":1}],"oa_status":"closed","license":"https://journals.sagepub.com/page/policies/text-and-data-mining-license","oa_locations":[{"url":"https://journals.sagepub.com/doi/full-xml/10.1089/hum.2021.283","host_type":"publisher"},{"url":"https://journals.sagepub.com/doi/pdf/10.1089/hum.2021.283","host_type":"publisher"},{"url":"https://doi.org/10.1089/hum.2021.283","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34963339","host_type":"repository"}],"fields_of_study":["CRISPR and Genetic Engineering","Innovation and Socioeconomic Development","Virus-based gene therapy research"],"mesh_terms":["Gene Editing","Endonucleases","Clustered Regularly Interspaced Short Palindromic Repeats","CRISPR-Cas Systems"],"keywords":["CRISPR","Genome editing","Palindrome","Computational biology","Cas9","Genome","Biology","Gene","Computer science","Genetics","Off-target Effects","Crispr/cas Nucleases","Base Editors","Prime Editors","Rna Base Editors"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T06:05:57.007589Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}