{"doi":"10.1089/cell.2019.0083","title":"Intermediate Standstill Clones Trapped in the Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells","abstract":null,"journal":"Cellular Reprogramming","year":2020,"id":46624,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":215930,"name":"Yebo Wang","orcid":null,"position":1,"is_corresponding":false},{"id":340,"name":"Ye Zhang","orcid":"0009-0000-4883-8097","position":2,"is_corresponding":false},{"id":215931,"name":"Limengmeng Wang","orcid":null,"position":3,"is_corresponding":false},{"id":54947,"name":"He Huang","orcid":"0000-0001-6998-8131","position":4,"is_corresponding":false},{"id":169696,"name":"Lifei Zhang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Intermediate Standstill Clones Trapped in the Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells","abstract":"Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lines originating from cells that have exited the normal reprogramming route at an early time point. Analysis of the associated global gene expression changes between iPSCs and piPSCs may help understand the barriers to reprogramming. In our study, human fibroblasts were transduced with the four classic transcription factors, OCT4, SOX2, KLF4, and C-MYC. Only a few cells were completely reprogrammed to a fully pluripotent state. Instead, we obtained more number of intermediate standstill clones than human-induced pluripotent stem cells (hiPSCs) during reprogramming. We studied the genome-wide expression profiles of two different fibroblasts, five intermediate standstill clones, and two iPSCs derived from the two fibroblasts. Hierarchical clustering and principal component analysis demonstrated that intermediate standstill clones were on the way to becoming hiPSCs. A remarkable difference in the expression of genes related to cancer and cell adhesion pathway was observed between the intermediate standstill clones and iPSCs. These observations suggest that some cells may become trapped in partially reprogrammed states.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32182120","pmcid":null,"openalex_id":"https://openalex.org/W3012348180","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.02171862,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"https://www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/","oa_locations":[{"url":"https://www.liebertpub.com/doi/pdf/10.1089/cell.2019.0083","host_type":"journal"},{"url":"https://www.liebertpub.com/doi/pdf/10.1089/cell.2019.0083","host_type":"BRONZE"},{"url":"https://www.liebertpub.com/doi/pdf/10.1089/cell.2019.0083","host_type":"publisher"},{"url":"https://www.liebertpub.com/doi/full-xml/10.1089/cell.2019.0083","host_type":"publisher"},{"url":"https://doi.org/10.1089/cell.2019.0083","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/32182120","host_type":"repository"}],"fields_of_study":["Pluripotent Stem Cells Research","CRISPR and Genetic Engineering","Animal Genetics and Reproduction","Biology","Medicine","Cell Differentiation","Cell Line","Cells, Cultured","Cellular Reprogramming","DNA-Binding Proteins","Fibroblasts","Gene Expression Regulation, Developmental","Genome-Wide Association Study","Humans","Induced Pluripotent Stem Cells","Kruppel-Like Factor 4","Kruppel-Like Transcription Factors","Octamer Transcription Factor-3","SOXB1 Transcription Factors","Transcription Factors","Transcriptome"],"mesh_terms":["Kruppel-Like Factor 4","Cell Differentiation","Cell Line","Cells, Cultured","DNA-Binding Proteins","Fibroblasts","Humans","Transcription Factors","Gene Expression Regulation, Developmental","Octamer Transcription Factor-3","Kruppel-Like Transcription Factors","Genome-Wide Association Study","SOXB1 Transcription Factors","Induced Pluripotent Stem Cells","Transcriptome","Cellular Reprogramming"],"keywords":["Reprogramming","Induced pluripotent stem cell","SOX2","KLF4","Biology","Somatic cell","Regenerative medicine","Cell biology","Stem cell","Genetics","Embryonic stem cell","Gene","Ips","Intermediate Standstill Clones"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-12T04:03:40.700054Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}