{"doi":"10.1089/bio.2022.0053","title":"The Influence of Preanalytical Biospecimen Handling on the Measurement of B Vitamers, Amino Acids, and Other Metabolites in Blood","abstract":"Introduction: Sample handling can influence biomarker measurement and introduce variability when combining data from multiple studies or study sites. To inform the development of blood collection protocols within a multisite cohort study, we directly quantified concentrations of 54 biomarkers in blood samples subjected to different handling conditions. Materials and Methods: We obtained serum, lithium heparin plasma, and EDTA plasma from 20 adult volunteers. Tubes of chilled whole blood were either centrifuged and processed within 2 hours of collection (the “reference standard”) or were stored with cool packs for 24 or 48 hours; centrifuged before and/or after this delay; or collected in tubes with/without gel separators. We used linear mixed models with random intercepts to estimate geometric mean concentrations and relative percent differences across the conditions. Results: Compared to the reference standard tubes, concentrations of many biomarkers changed after processing delays, but changes were often small. In serum, we observed large differences for B vitamers, glutamic acid (37% and 73% increases with 24- and 48-hour delays, respectively), glycine (12% and 23% increases), serine (16% and 27% increases), and acetoacetate (−19% and −26% decreases). Centrifugation timing and separator tube use did not affect concentrations of most biomarkers. Conclusion: Sample handling should be consistent across samples within an analysis. The length of processing delays should be recorded and accounted for when this is not feasible.","journal":"Biopreservation and Biobanking","year":2023,"id":374045,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8442,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":240083,"name":"Stephanie J. Weinstein","orcid":"0000-0002-3834-1535","position":1,"is_corresponding":false},{"id":329158,"name":"Paul S. Albert","orcid":"0000-0003-1658-1068","position":2,"is_corresponding":false},{"id":305533,"name":"Amanda Black","orcid":"0000-0002-2245-3428","position":3,"is_corresponding":false},{"id":34362,"name":"Michelle Brotzman","orcid":null,"position":4,"is_corresponding":false},{"id":1136082,"name":"Norma A. Diaz-Mayoral","orcid":null,"position":5,"is_corresponding":false},{"id":436755,"name":"Nicole Gerlanc","orcid":null,"position":6,"is_corresponding":false},{"id":241825,"name":"Wen‐Yi Huang","orcid":"0000-0002-4440-3368","position":7,"is_corresponding":false},{"id":241829,"name":"Joshua N. Sampson","orcid":"0000-0003-2875-3365","position":8,"is_corresponding":false},{"id":898304,"name":"Alaina H. Shreves","orcid":"0000-0002-0127-4391","position":9,"is_corresponding":false},{"id":392601,"name":"Per Magne Ueland","orcid":"0000-0002-1903-0571","position":10,"is_corresponding":false},{"id":449976,"name":"Kathleen Wyatt","orcid":null,"position":11,"is_corresponding":false},{"id":50161,"name":"Nicolas Wentzensen","orcid":"0000-0003-1251-0836","position":12,"is_corresponding":false},{"id":217938,"name":"Christian C. Abnet","orcid":"0000-0002-3008-7843","position":13,"is_corresponding":false},{"id":428011,"name":"Kara A. Michels","orcid":"0000-0003-2431-2079","position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":null,"created_at":"2026-07-19T01:16:11.341687Z","pmid":"36622937","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}