{"doi":"10.1089/aid.1994.10.295","title":"A Sensitive Reporter Cell Line for HIV-1\n                    <i>tat</i>\n                    Activity, HIV-1 Inhibitors, and T Cell Activation Effects","abstract":"<jats:p>\n                    The production and characterization of a cell line for quantitative HIV-1 Tat function and T cell activation assays is described. 1G5 is a clonal cell line derived from Jurkat T cells stably transfected with a luciferase gene driven by an HIV-1 long terminal repeat (HIV-LTR). The 1G5 clone was selected for low basal luciferase activity, susceptibility to HIV infection, and high responsiveness to Tat and T cell activation signals. A 10 to 1000-fold increase in luciferase activity after transfection or infection with\n                    <jats:italic toggle=\"yes\">tat</jats:italic>\n                    -expressing vectors or HIV was observed. Equivalent levels of expression were detected after stimulation with T cell mitogens. The characteristics of 1G5 make it a valuable reagent for studies of HIV infection, HIV regulatory agents, and other T cell or HIV -activating factors, and for screening potential anti-HIV therapeutic agents.\n                  </jats:p>","journal":"AIDS Research and Human Retroviruses","year":1994,"id":15245,"datarank":8.748076773902394,"base_score":4.875197323201151,"endowment":4.875197323201151,"self_citation_contribution":0.7312795984801728,"citation_network_contribution":8.016797175422221,"self_endowment_contribution":0.7312795984801728,"citer_contribution":8.016797175422221,"corpus_percentile":null,"corpus_rank":null,"citation_count":130,"citer_count":130,"citers_with_citation_signal":119,"citers_with_endowment":119,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":117070,"name":"Javier Chinen","orcid":null,"position":1,"is_corresponding":false},{"id":117071,"name":"Larry Donehower","orcid":null,"position":2,"is_corresponding":false},{"id":117072,"name":"Dorothy E. Lewis","orcid":null,"position":3,"is_corresponding":false},{"id":117073,"name":"John W. Belmont","orcid":null,"position":4,"is_corresponding":false},{"id":117069,"name":"Estuardo Aguilar-Cordova","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.875197323201151,"endowment":4.875197323201151,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8018390","pmcid":null,"openalex_id":"https://openalex.org/W2023243934","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"NIAID U01-A130243","title":null}],"total_grants":1,"fwci":1.3015,"citation_percentile":0.76882845,"influential_citations":15,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":4},{"year":2014,"count":1},{"year":2015,"count":5},{"year":2016,"count":2},{"year":2017,"count":4},{"year":2018,"count":5},{"year":2019,"count":2},{"year":2020,"count":5},{"year":2021,"count":6},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":2},{"year":2026,"count":2}],"oa_status":"closed","license":"https://journals.sagepub.com/page/policies/text-and-data-mining-license","oa_locations":[{"url":"https://journals.sagepub.com/doi/pdf/10.1089/aid.1994.10.295","host_type":"publisher"},{"url":"https://doi.org/10.1089/aid.1994.10.295","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8018390","host_type":"repository"}],"fields_of_study":["HIV Research and Treatment","Immune Cell Function and Interaction","T-cell and B-cell Immunology","Medicine","Biology","Amino Acid Sequence","Cell Line","DNA Primers","Gene Products, tat","Genes, tat","HIV-1","Humans","Luciferases","Lymphocyte Activation","Molecular Sequence Data","T-Lymphocytes","Zidovudine","tat Gene Products, Human Immunodeficiency Virus"],"mesh_terms":["Amino Acid Sequence","Cell Line","Humans","Luciferases","Lymphocyte Activation","Molecular Sequence Data","T-Lymphocytes","Zidovudine","HIV-1","Gene Products, tat","Genes, tat","DNA Primers","tat Gene Products, Human Immunodeficiency Virus"],"keywords":["Jurkat cells","Luciferase","Transfection","clone (Java method)","Cell culture","Biology","Lentivirus","Molecular biology","HIV Long Terminal Repeat","Cell","Reporter gene","Virology","T cell","Human immunodeficiency virus (HIV)","Long terminal repeat","Gene expression","Gene","Immunology","Viral disease","Biochemistry","Genetics","Immune system"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T16:57:02.519154Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}