{"doi":"10.1089/aid.1993.9.825","title":"Activation of the Human Immunodeficiency Virus Type 1 Long Terminal Repeat by BTEB, a GC Box-Binding Transcription Factor","abstract":"<jats:p>BTEB, a GC box-binding transcription factor, was tested for its ability to activate the human immunodeficiency virus type 1 long terminal repeat (HIV-1 LTR). An electrophoretic mobility shift assay demonstrated specific binding of BTEB to GC boxes of the HIV-1 LTR. When a BTEB expression vector was cotransfected into A3.01 cells with a fusion gene of HIV-1 LTR and chloramphenicol acetyltransferase (CAT) structural gene, the CAT activity was increased. This increase was accompanied by an increase in the content of CAT mRNA. Transcriptional activity of the HIV-1 LTR, stimulated by Tat, was further enhanced by the expression of BTEB. BTEB also activated the LTR activity in cooperation with phorbol 12-myristate 13-acetate. Northern blot analysis showed that various T cell and macrophage/monocyte cell lines expressed the BTEB mRNA to a level comparable with that of Sp1, another GC box-binding transcription factor. These results suggest that BTEB, like Sp1, is involved in transcriptional activation of the HIV-1 LTR.</jats:p>","journal":"AIDS Research and Human Retroviruses","year":1993,"id":15359,"datarank":0.9804147125490136,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.5310548715159149,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.5310548715159149,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":18,"citers_with_citation_signal":16,"citers_with_endowment":16,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":117477,"name":"Akiko Mizuno","orcid":null,"position":1,"is_corresponding":false},{"id":117478,"name":"Yoshiaki Fujii-Kuriyama","orcid":null,"position":2,"is_corresponding":false},{"id":116221,"name":"Masanori Hayami","orcid":null,"position":3,"is_corresponding":false},{"id":117476,"name":"Hiroaki Imataka","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.995732273553991,"endowment":2.995732273553991,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8257632","pmcid":null,"openalex_id":"https://openalex.org/W2069658259","authors":[],"funders":[],"total_grants":0,"fwci":1.1214,"citation_percentile":0.81515244,"influential_citations":1,"citation_trend":[{"year":2015,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":4},{"year":2025,"count":1}],"oa_status":"closed","license":"https://journals.sagepub.com/page/policies/text-and-data-mining-license","oa_locations":[{"url":"https://journals.sagepub.com/doi/pdf/10.1089/aid.1993.9.825","host_type":"publisher"},{"url":"https://doi.org/10.1089/aid.1993.9.825","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8257632","host_type":"repository"}],"fields_of_study":["Plant Virus Research Studies","Cancer-related gene regulation","HIV Research and Treatment","Biology","Medicine","Base Sequence","Binding Sites","DNA-Binding Proteins","Gene Products, tat","HIV Long Terminal Repeat","HIV-1","Humans","Kruppel-Like Transcription Factors","Molecular Sequence Data","NF-kappa B","T-Lymphocytes","Tetradecanoylphorbol Acetate","Transcription Factors","Transcription, Genetic","Tumor Cells, Cultured","Virus Activation","Zinc Fingers","tat Gene Products, Human Immunodeficiency Virus"],"mesh_terms":["Base Sequence","Binding Sites","DNA-Binding Proteins","Humans","Molecular Sequence Data","T-Lymphocytes","Tetradecanoylphorbol Acetate","Transcription Factors","Transcription, Genetic","Tumor Cells, Cultured","Virus Activation","HIV-1","Gene Products, tat","HIV Long Terminal Repeat","NF-kappa B","Zinc Fingers","Kruppel-Like Transcription Factors","tat Gene Products, Human Immunodeficiency Virus"],"keywords":["Long terminal repeat","Chloramphenicol acetyltransferase","Molecular biology","HIV Long Terminal Repeat","Biology","Transcription factor","Northern blot","Transcription (linguistics)","Gene expression","Electrophoretic mobility shift assay","Gene","Promoter","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T17:24:40.611596Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}