{"doi":"10.1089/adt.2011.415","title":"Comparison of Cell Expression Formats for the Characterization of GABA\n                    <sub>A</sub>\n                    Channels Using a Microfluidic Patch Clamp System","abstract":null,"journal":"ASSAY and Drug Development Technologies","year":2012,"id":654359,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":415783,"name":"Peter D. Yim","orcid":"0000-0003-1670-7599","position":1,"is_corresponding":false},{"id":1707763,"name":"Nina Yuan","orcid":null,"position":2,"is_corresponding":false},{"id":1078532,"name":"Juliette Johnson","orcid":null,"position":3,"is_corresponding":false},{"id":70883,"name":"James M. Cook","orcid":"0000-0003-3705-8540","position":4,"is_corresponding":false},{"id":134882,"name":"Steve Smith","orcid":null,"position":5,"is_corresponding":false},{"id":1707764,"name":"Cristian Ionescu-Zanetti","orcid":null,"position":6,"is_corresponding":false},{"id":1707765,"name":"Zhi-Jian Wang","orcid":null,"position":7,"is_corresponding":false},{"id":524994,"name":"Leggy A. Arnold","orcid":"0000-0003-1411-1572","position":8,"is_corresponding":false},{"id":485294,"name":"Charles W. Emala","orcid":"0000-0003-0248-4662","position":9,"is_corresponding":false},{"id":114358,"name":"Qin Chen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Comparison of Cell Expression Formats for the Characterization of GABA\n                    <sub>A</sub>\n                    Channels Using a Microfluidic Patch Clamp System","abstract":"Ensemble recording and microfluidic perfusion are recently introduced techniques aimed at removing the laborious nature and low recording success rates of manual patch clamp. Here, we present assay characteristics for these features integrated into one automated electrophysiology platform as applied to the study of GABA(A) channels. A variety of cell types and methods of GABA(A) channel expression were successfully studied (defined as I(GABA)>500 pA), including stably transfected human embryonic kidney (HEK) cells expressing α(1)β(3)γ(2) GABA(A) channels, frozen ready-to-assay (RTA) HEK cells expressing α(1)β(3)γ(2) or α(3)β(3)γ(2) GABA(A) channels, transiently transfected HEK293T cells expressing α(1)β(3)γ(2) GABA(A) channels, and immortalized cultures of human airway smooth muscle cells endogenously expressing GABA(A) channels. Current measurements were successfully studied in multiple cell types with multiple modes of channel expression in response to several classic GABA(A) channel agonists, antagonists, and allosteric modulators. We obtained success rates above 95% for transiently or stably transfected HEK cells and frozen RTA HEK cells expressing GABA(A) channels. Tissue-derived immortalized cultures of airway smooth muscle cells exhibited a slightly lower recording success rate of 75% using automated patch, which was much higher than the 5% success rate using manual patch clamp technique by the same research group. Responses to agonists, antagonists, and allosteric modulators compared well to previously reported manual patch results. The data demonstrate that both the biophysics and pharmacologic characterization of GABA(A) channels in a wide variety of cell formats can be performed using this automated patch clamp system.","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22574655","pmcid":"PMC3419985","openalex_id":"https://openalex.org/W2147804649","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM065281","title":null},{"funder_name":"PHS HHS","grant_id":"MIL100371","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"R01 NS076517","title":null},{"funder_name":"NIMH NIH HHS","grant_id":"R01 MH096463","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM008464","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM065281","title":null}],"total_grants":6,"fwci":0.2287,"citation_percentile":0.47304522,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2015,"count":1},{"year":2016,"count":2},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2022,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3419985","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3419985","host_type":"repository"},{"url":"http://www.liebertpub.com/doi/pdf/10.1089/adt.2011.415","host_type":"publisher"},{"url":"https://doi.org/10.1089/adt.2011.415","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22574655","host_type":"repository"},{"url":"http://europepmc.org/articles/PMC3419985","host_type":"repository"}],"fields_of_study":["Neuroscience and Neural Engineering","Neuroscience and Neuropharmacology Research","Ion channel regulation and function","Benzodiazepines","Bicuculline","Diazepam","GABA Agents","GABA Agonists","GABA Antagonists","GABA Modulators","Gene Expression","HEK293 Cells","High-Throughput Screening Assays","Humans","Imidazoles","Ion Channel Gating","Microfluidic Analytical Techniques","Muscimol","Patch-Clamp Techniques","Picrotoxin","Receptors, GABA-A","Recombinant Proteins","Transfection"],"mesh_terms":["Benzodiazepines","Bicuculline","Diazepam","Humans","Imidazoles","Muscimol","Picrotoxin","Receptors, GABA-A","Recombinant Proteins","Transfection","Ion Channel Gating","Gene Expression","Patch-Clamp Techniques","GABA Agents","GABA Agonists","GABA Antagonists","GABA Modulators","Microfluidic Analytical Techniques","High-Throughput Screening Assays","HEK293 Cells"],"keywords":["HEK 293 cells","Patch clamp","Transfection","Immortalised cell line","Ion channel","Electrophysiology","Cell biology","Cell culture","Chemistry","Allosteric regulation","gamma-Aminobutyric acid","Cell","Biophysics","Molecular biology","Neuroscience","Biology","Biochemistry","Receptor","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T06:05:54.508185Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}