{"doi":"10.1089/adt.2010.0301","title":"A Miniaturized Homogenous Assay of Mitochondrial Membrane Potential","abstract":"<jats:p>Mitochondrial dysfunction is increasingly associated with disease states. These organelles, responsible for adenosine triphosphate production, have been targeted for improved function in such diseases as Parkinson's, Alzheimer's, type 2 diabetes, and sarcopenia. In addition, the importance of determining if a clinical drug candidate adversely effects mitochondria function, which could lead to overt toxicity, has been recognized. Hence, assays that measure mitochondria activity have become essential in early stage drug development. Limitations of current assays that measure mitochondria membrane potential have prohibited the high-throughput performance necessary to screen current chemical space. Here, we describe a homogeneous assay to measure mitochondria membrane potential that can utilize either adherent or suspension cell types. The assay has been miniaturized to 1,536-well plate format, and was used to perform a fully automated robotic high-throughput screen of a small molecule chemical library.</jats:p>","journal":"ASSAY and Drug Development Technologies","year":2011,"id":676045,"datarank":0.40918061159833635,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.11729408924003931,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.11729408924003931,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":6,"citers_with_citation_signal":5,"citers_with_endowment":5,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1766445,"name":"Kelli J. Solly","orcid":null,"position":1,"is_corresponding":false},{"id":1766446,"name":"Laszlo Kiss","orcid":null,"position":2,"is_corresponding":false},{"id":118558,"name":"Douglas J. MacNeil","orcid":null,"position":3,"is_corresponding":false},{"id":1766447,"name":"Charles I. Rosenblum","orcid":null,"position":4,"is_corresponding":false},{"id":1766444,"name":"Aurawan Vongs","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Miniaturized Homogenous Assay of Mitochondrial Membrane Potential","abstract":"<jats:p>Mitochondrial dysfunction is increasingly associated with disease states. These organelles, responsible for adenosine triphosphate production, have been targeted for improved function in such diseases as Parkinson's, Alzheimer's, type 2 diabetes, and sarcopenia. In addition, the importance of determining if a clinical drug candidate adversely effects mitochondria function, which could lead to overt toxicity, has been recognized. Hence, assays that measure mitochondria activity have become essential in early stage drug development. Limitations of current assays that measure mitochondria membrane potential have prohibited the high-throughput performance necessary to screen current chemical space. Here, we describe a homogeneous assay to measure mitochondria membrane potential that can utilize either adherent or suspension cell types. The assay has been miniaturized to 1,536-well plate format, and was used to perform a fully automated robotic high-throughput screen of a small molecule chemical library.</jats:p>","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21294696","pmcid":null,"openalex_id":"https://openalex.org/W2128773761","authors":[],"funders":[],"total_grants":0,"fwci":0.1216,"citation_percentile":0.50788954,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2021,"count":2},{"year":2024,"count":1}],"oa_status":"closed","license":"https://journals.sagepub.com/page/policies/text-and-data-mining-license","oa_locations":[{"url":"https://journals.sagepub.com/doi/pdf/10.1089/adt.2010.0301","host_type":"publisher"},{"url":"https://journals.sagepub.com/doi/full-xml/10.1089/adt.2010.0301","host_type":"publisher"},{"url":"https://doi.org/10.1089/adt.2010.0301","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21294696","host_type":"repository"}],"fields_of_study":["Mitochondrial Function and Pathology","Electrochemical sensors and biosensors","Metabolism and Genetic Disorders","Animals","Biological Assay","CHO Cells","Carbonyl Cyanide m-Chlorophenyl Hydrazone","Coloring Agents","Cricetinae","Drug Evaluation, Preclinical","Drug-Related Side Effects and Adverse Reactions","High-Throughput Screening Assays","Humans","Jurkat Cells","Luminescent Measurements","Membrane Potential, Mitochondrial","Membrane Potentials","Miniaturization","Mitochondria","Proton Ionophores","Rhodamines","Time Factors"],"mesh_terms":["Animals","Biological Assay","Carbonyl Cyanide m-Chlorophenyl Hydrazone","Drug Evaluation, Preclinical","Coloring Agents","Cricetinae","Humans","Luminescent Measurements","Membrane Potentials","Miniaturization","Mitochondria","Rhodamines","Time Factors","CHO Cells","Jurkat Cells","Membrane Potential, Mitochondrial","High-Throughput Screening Assays","Proton Ionophores","Drug-Related Side Effects and Adverse Reactions"],"keywords":["Mitochondrion","Drug discovery","Chemical library","Organelle","Adenosine triphosphate","Membrane potential","Cell biology","Chemistry","High-throughput screening","Biology","Biochemistry","Small molecule"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T02:00:55.664864Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}