{"doi":"10.1088/1758-5090/ae302c","title":"Real-time imaging of platelet dynamics in engineered arterial, venous, and cancer-associated thrombotic microenvironments","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Thrombosis is a leading cause of cardiovascular morbidity and mortality, driven by platelet-mediated mechanisms common across distinct vascular environments. However, the dynamical behavior of platelets during thrombogenesis remains poorly understood due to the lack of a comprehensive analytical framework. Here we present a physiologically relevant, imaging-integrated thrombosis-on-a-chip platform that enables real-time quantitative analysis of platelet dynamics during thrombogenesis under arterial, venous, and cancer-associated conditions. The system incorporates endothelialized 3D-printed vascular geometries into a closed-loop whole-blood perfusion circuit that replicates native hemodynamic and cellular microenvironments. Multimodal imaging captures the spatiotemporal evolution of thrombus formation and shows how hydrodynamic forces, endothelial dysfunction, and tumor-derived factors drive distinct thrombotic signatures. Notably, platelet-endothelium adhesion and circulating platelet aggregation are identified as mechanistically distinct yet closely linked processes, each uniquely modulated by vascular context. This platform offers a robust framework for dissecting thrombogenesis and advancing antithrombotic and cancer-associated thrombosis research.</jats:p>","journal":"Biofabrication","year":2026,"id":629678,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":642709,"name":"Yuqi Zhou","orcid":"0000-0002-1206-0049","position":1,"is_corresponding":false},{"id":1630900,"name":"Hui Min Tay","orcid":null,"position":2,"is_corresponding":false},{"id":1630901,"name":"Huidong Wang","orcid":null,"position":3,"is_corresponding":false},{"id":473343,"name":"Junyu Chen","orcid":"0000-0001-9659-0225","position":4,"is_corresponding":false},{"id":1630902,"name":"Yingdong Luo","orcid":null,"position":5,"is_corresponding":false},{"id":643740,"name":"Hongqian Zhang","orcid":null,"position":6,"is_corresponding":false},{"id":997209,"name":"Chenqi Zhang","orcid":"0000-0003-3241-821X","position":7,"is_corresponding":false},{"id":643737,"name":"Yuma Ibayashi","orcid":null,"position":8,"is_corresponding":false},{"id":1630903,"name":"Petra Paiè","orcid":null,"position":9,"is_corresponding":false},{"id":642707,"name":"Masako Nishikawa","orcid":"0000-0002-3840-767X","position":10,"is_corresponding":false},{"id":1183421,"name":"Yohei Mineharu","orcid":"0000-0002-6346-3999","position":11,"is_corresponding":false},{"id":1630904,"name":"Eriko Morishita","orcid":"0000-0001-9116-3363","position":12,"is_corresponding":false},{"id":642720,"name":"Yutaka Yatomi","orcid":"0000-0003-1719-4297","position":13,"is_corresponding":false},{"id":1630905,"name":"Han Wei Hou","orcid":"0000-0001-6631-6321","position":14,"is_corresponding":false},{"id":642721,"name":"Keisuke Goda","orcid":"0000-0001-6302-6038","position":15,"is_corresponding":false},{"id":642718,"name":"Yunjie Deng","orcid":"0000-0003-4118-9068","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Real-time imaging of platelet dynamics in engineered arterial, venous, and cancer-associated thrombotic microenvironments","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Thrombosis is a leading cause of cardiovascular morbidity and mortality, driven by platelet-mediated mechanisms common across distinct vascular environments. However, the dynamical behavior of platelets during thrombogenesis remains poorly understood due to the lack of a comprehensive analytical framework. Here we present a physiologically relevant, imaging-integrated thrombosis-on-a-chip platform that enables real-time quantitative analysis of platelet dynamics during thrombogenesis under arterial, venous, and cancer-associated conditions. The system incorporates endothelialized 3D-printed vascular geometries into a closed-loop whole-blood perfusion circuit that replicates native hemodynamic and cellular microenvironments. Multimodal imaging captures the spatiotemporal evolution of thrombus formation and shows how hydrodynamic forces, endothelial dysfunction, and tumor-derived factors drive distinct thrombotic signatures. Notably, platelet-endothelium adhesion and circulating platelet aggregation are identified as mechanistically distinct yet closely linked processes, each uniquely modulated by vascular context. This platform offers a robust framework for dissecting thrombogenesis and advancing antithrombotic and cancer-associated thrombosis research.</jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41429050","pmcid":null,"openalex_id":"https://openalex.org/W7116858438","authors":[],"funders":[{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP23hma922009 (M.N.","title":null},{"funder_name":"Japan Society for the Promotion of Science","grant_id":"9H05633 (K.G.)","title":null},{"funder_name":"Japan Science and Technology Agency","grant_id":"JPMJSP2108 (Y.D.)","title":null},{"funder_name":"European Union","grant_id":"101235654 (P.P.","title":null},{"funder_name":"KAKETSUKEN","grant_id":"","title":null},{"funder_name":"White Rock Foundation","grant_id":"","title":null},{"funder_name":"Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering","grant_id":"","title":null},{"funder_name":"Charitable Trust Laboratory Medicine Research Foundation of Japan","grant_id":"","title":null}],"total_grants":8,"fwci":2.119,"citation_percentile":0.89875128,"influential_citations":0,"citation_trend":[{"year":2026,"count":2}],"oa_status":"closed","license":"https://iopscience.iop.org/info/page/text-and-data-mining","oa_locations":[{"url":"https://iopscience.iop.org/article/10.1088/1758-5090/ae302c","host_type":"publisher"},{"url":"https://iopscience.iop.org/article/10.1088/1758-5090/ae302c/pdf","host_type":"publisher"},{"url":"https://doi.org/10.1088/1758-5090/ae302c","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41429050","host_type":"repository"},{"url":"https://hdl.handle.net/10356/211614","host_type":"repository"},{"url":"https://hdl.handle.net/11311/1311198","host_type":"repository"}],"fields_of_study":["Platelet Disorders and Treatments","Blood properties and coagulation","Angiogenesis and VEGF in Cancer"],"mesh_terms":["Arteries","Blood Platelets","Humans","Neoplasms","Platelet Aggregation","Thrombosis","Veins","Tissue Engineering","Lab-On-A-Chip Devices","Tumor Microenvironment"],"keywords":["Thrombus","Platelet","Antithrombotic","Thrombosis","Dynamics (music)","Platelet activation","Hemodynamics","Platelet adhesiveness","High-throughput Imaging","Primary Human Endothelial Cells","Platelet Dynamics","Hydrodynamic Shear Stress","Microfluidic Thrombogenesis Platform"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T19:23:43.211907Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}