{"doi":"10.1088/1361-6463/aab8c8","title":"Effects of nanosecond pulsed electric fields (nsPEFs) on the human fungal pathogen\n                    <i>Candida albicans:</i>\n                    an\n                    <i>in vitro</i>\n                    study","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    <jats:italic>Candida albicans</jats:italic>\n                    is the leading human fungal pathogen that causes many life-threatening infections. Notably, the current clinical trial data indicate that Candida species shows the emerging resistance to anti-fungal drugs. The aim of this study was to evaluate the antifungal effects of nanosecond pulsed electric fields (nsPEFs) as a novel drug-free strategy\n                    <jats:italic>in vitro</jats:italic>\n                    . In this study, we investigated the inactivation and permeabilization effects of\n                    <jats:italic>C. albicans</jats:italic>\n                    under different nsPEFs exposure conditions (100 pulses, 100 ns in duration, intensities of 20, 40 kV cm\n                    <jats:sup>−1</jats:sup>\n                    ). Cell death was studied by annexin-V and propidium iodide staining. The changes of intracellular Ca\n                    <jats:sup>2+</jats:sup>\n                    concentration after nsPEFs treatment were observed using Fluo-4 AM. Results show that\n                    <jats:italic>C. albicans</jats:italic>\n                    cells and biofilms were both obviously inhibited and destroyed after nsPEFs treatment. Furthermore,\n                    <jats:italic>C. albicans</jats:italic>\n                    cells were significantly permeabilized after nsPEFs treatment. Additionally, nsPEFs exposure led to a large amount of DNA and protein leakage. Importantly, nsPEFs induced a field strength-dependent apoptosis in\n                    <jats:italic>C. albicans</jats:italic>\n                    cells. Further experiments revealed that Ca\n                    <jats:sup>2+</jats:sup>\n                    involved in nsPEFs induced\n                    <jats:italic>C. albicans</jats:italic>\n                    apoptosis. In conclusion, this proof-of-concept study provides a potential alternative drug-free strategy for killing pathogenic Candida species.\n                  </jats:p>","journal":"Journal of Physics D: Applied Physics","year":2018,"id":602168,"datarank":0.3958585994422889,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.0,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1544290,"name":"Jie Dang","orcid":null,"position":1,"is_corresponding":false},{"id":1028202,"name":"Kaile Wang","orcid":"0000-0003-2249-1026","position":2,"is_corresponding":false},{"id":956838,"name":"Jue Zhang","orcid":"0000-0003-0440-1357","position":3,"is_corresponding":false},{"id":263768,"name":"Jing Fang","orcid":"0000-0002-4950-626X","position":4,"is_corresponding":false},{"id":1544288,"name":"Jinsong Guo","orcid":"0000-0002-7379-4708","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Effects of nanosecond pulsed electric fields (nsPEFs) on the human fungal pathogen\n                    <i>Candida albicans:</i>\n                    an\n                    <i>in vitro</i>\n                    study","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    <jats:italic>Candida albicans</jats:italic>\n                    is the leading human fungal pathogen that causes many life-threatening infections. Notably, the current clinical trial data indicate that Candida species shows the emerging resistance to anti-fungal drugs. The aim of this study was to evaluate the antifungal effects of nanosecond pulsed electric fields (nsPEFs) as a novel drug-free strategy\n                    <jats:italic>in vitro</jats:italic>\n                    . In this study, we investigated the inactivation and permeabilization effects of\n                    <jats:italic>C. albicans</jats:italic>\n                    under different nsPEFs exposure conditions (100 pulses, 100 ns in duration, intensities of 20, 40 kV cm\n                    <jats:sup>−1</jats:sup>\n                    ). Cell death was studied by annexin-V and propidium iodide staining. The changes of intracellular Ca\n                    <jats:sup>2+</jats:sup>\n                    concentration after nsPEFs treatment were observed using Fluo-4 AM. Results show that\n                    <jats:italic>C. albicans</jats:italic>\n                    cells and biofilms were both obviously inhibited and destroyed after nsPEFs treatment. Furthermore,\n                    <jats:italic>C. albicans</jats:italic>\n                    cells were significantly permeabilized after nsPEFs treatment. Additionally, nsPEFs exposure led to a large amount of DNA and protein leakage. Importantly, nsPEFs induced a field strength-dependent apoptosis in\n                    <jats:italic>C. albicans</jats:italic>\n                    cells. Further experiments revealed that Ca\n                    <jats:sup>2+</jats:sup>\n                    involved in nsPEFs induced\n                    <jats:italic>C. albicans</jats:italic>\n                    apoptosis. In conclusion, this proof-of-concept study provides a potential alternative drug-free strategy for killing pathogenic Candida species.\n                  </jats:p>","is_dataset_classified":null,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":"https://openalex.org/W2905407585","authors":[],"funders":[],"total_grants":0,"fwci":0.7797,"citation_percentile":0.72083854,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":4},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":"https://iopscience.iop.org/info/page/text-and-data-mining","oa_locations":[{"url":"http://stacks.iop.org/0022-3727/51/i=18/a=185402?key=crossref.56f33137576ebebda0adae046d86e191","host_type":"publisher"},{"url":"http://iopscience.iop.org/article/10.1088/1361-6463/aab8c8/pdf","host_type":"publisher"},{"url":"http://stacks.iop.org/0022-3727/51/i=18/a=185402/pdf","host_type":"publisher"},{"url":"http://iopscience.iop.org/article/10.1088/1361-6463/aab8c8","host_type":"publisher"},{"url":"https://iopscience.iop.org/article/10.1088/1361-6463/aab8c8","host_type":"publisher"},{"url":"https://iopscience.iop.org/article/10.1088/1361-6463/aab8c8/pdf","host_type":"publisher"},{"url":"https://doi.org/10.1088/1361-6463/aab8c8","host_type":"journal"}],"fields_of_study":["Microbial Inactivation Methods","bioluminescence and chemiluminescence research","Transgenic Plants and Applications"],"mesh_terms":[],"keywords":["Candida albicans","Fungal pathogen","In vitro","Nanosecond","Pathogen","Microbiology","Human pathogen","Chemistry","Biology","Optics","Physics","Biochemistry","Laser"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T18:31:13.138862Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}