{"doi":"10.1084/jem.20222129","title":"CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes","abstract":"Dendritic cells (DCs) and monocytes capture, transport, and present antigen to cognate T cells in the draining lymph nodes (LNs) in a CCR7-dependent manner. Since only migratory DCs express this chemokine receptor, it is unclear how monocytes reach the LN. In steady-state and following inhalation of several PAMPs, scRNA-seq identified LN mononuclear phagocytes as monocytes, resident, or migratory type 1 and type 2 conventional (c)DCs, despite the downregulation of Xcr1, Clec9a, H2-Ab1, Sirpa, and Clec10a transcripts on migratory cDCs. Migratory cDCs, however, upregulated Ccr7, Ccl17, Ccl22, and Ccl5. Migratory monocytes expressed Ccr5, a high-affinity receptor for Ccl5. Using two tracking methods, we observed that both CD88hiCD26lomonocytes and CD88-CD26hi cDCs captured inhaled antigens in the lung and migrated to LNs. Antigen exposure in mixed-chimeric Ccl5-, Ccr2-, Ccr5-, Ccr7-, and Batf3-deficient mice demonstrated that while antigen-bearing DCs use CCR7 to reach the LN, monocytes use CCR5 to follow CCL5-secreting migratory cDCs into the LN, where they regulate DC-mediated immunity.","journal":"The Journal of Experimental Medicine","year":2023,"id":316412,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":104,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9473,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":303830,"name":"Anita Tewari","orcid":"0000-0001-6331-1701","position":1,"is_corresponding":false},{"id":858164,"name":"Xin Li","orcid":"0000-0002-2050-2628","position":2,"is_corresponding":false},{"id":719070,"name":"Arlind B. Mara","orcid":"0000-0001-9823-5243","position":3,"is_corresponding":false},{"id":289077,"name":"W. T. King","orcid":"0000-0003-2312-6207","position":4,"is_corresponding":false},{"id":297347,"name":"Sophie L. Gibbings","orcid":"0000-0001-9623-0705","position":5,"is_corresponding":false},{"id":1019943,"name":"Chinaza F. Nnam","orcid":"0000-0001-6230-6169","position":6,"is_corresponding":false},{"id":397493,"name":"Fred Kolling","orcid":"0000-0002-6178-9901","position":7,"is_corresponding":false},{"id":80095,"name":"Bart N. Lambrecht","orcid":"0000-0003-4376-6834","position":8,"is_corresponding":false},{"id":61572,"name":"Claudia Jakubzick","orcid":"0000-0002-3731-0198","position":9,"is_corresponding":false},{"id":576081,"name":"Kavita Rawat","orcid":"0000-0002-8302-9890","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-19T01:06:38.213358Z","pmid":"36946983","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}