{"doi":"10.1084/jem.20201387","title":"SARS-CoV-2 induces human plasmacytoid predendritic cell diversification via UNC93B and IRAK4","abstract":"Several studies have analyzed antiviral immune pathways in late-stage severe COVID-19. However, the initial steps of SARS-CoV-2 antiviral immunity are poorly understood. Here we have isolated primary SARS-CoV-2 viral strains and studied their interaction with human plasmacytoid predendritic cells (pDCs), a key player in antiviral immunity. We show that pDCs are not productively infected by SARS-CoV-2. However, they efficiently diversified into activated P1-, P2-, and P3-pDC effector subsets in response to viral stimulation. They expressed CD80, CD86, CCR7, and OX40 ligand at levels similar to influenza virus-induced activation. They rapidly produced high levels of interferon-α, interferon-λ1, IL-6, IP-10, and IL-8. All major aspects of SARS-CoV-2-induced pDC activation were inhibited by hydroxychloroquine. Mechanistically, SARS-CoV-2-induced pDC activation critically depended on IRAK4 and UNC93B1, as established using pDC from genetically deficient patients. Overall, our data indicate that human pDC are efficiently activated by SARS-CoV-2 particles and may thus contribute to type I IFN-dependent immunity against SARS-CoV-2 infection.","journal":"The Journal of Experimental Medicine","year":2021,"id":147996,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":139,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9591,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":104474,"name":"Lucie Bonnet‐Madin","orcid":null,"position":1,"is_corresponding":false},{"id":617474,"name":"Laurent Meertens","orcid":"0000-0003-2811-3675","position":2,"is_corresponding":false},{"id":104472,"name":"Léa Karpf","orcid":"0000-0001-6959-7528","position":3,"is_corresponding":false},{"id":629869,"name":"Justine Poirot","orcid":"0000-0002-6694-7796","position":4,"is_corresponding":false},{"id":57374,"name":"Shen-Ying Zhang","orcid":"0000-0002-9449-3672","position":5,"is_corresponding":false},{"id":622997,"name":"Capucine Pïcard","orcid":"0000-0001-8788-5056","position":6,"is_corresponding":false},{"id":104226,"name":"Anne Puel","orcid":"0000-0003-2603-0323","position":7,"is_corresponding":false},{"id":104229,"name":"Emmanuelle Jouanguy","orcid":"0000-0002-7358-9157","position":8,"is_corresponding":false},{"id":104140,"name":"Qian Zhang","orcid":"0000-0002-9040-3289","position":9,"is_corresponding":false},{"id":631247,"name":"Jérôme Le Goff","orcid":null,"position":10,"is_corresponding":false},{"id":629870,"name":"Jean‐Michel Molina","orcid":"0000-0001-8684-2214","position":11,"is_corresponding":false},{"id":629871,"name":"Constance Delaugerre","orcid":"0000-0001-6081-7993","position":12,"is_corresponding":false},{"id":57373,"name":"Jean-Laurent Casanova","orcid":"0000-0002-7782-4169","position":13,"is_corresponding":false},{"id":629872,"name":"Ali Amara","orcid":"0000-0002-0283-1815","position":14,"is_corresponding":false},{"id":629873,"name":"Vassili Soumelis","orcid":"0000-0003-1849-9834","position":15,"is_corresponding":false},{"id":104470,"name":"Fanny Onodi","orcid":"0000-0002-8429-4821","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-18T23:42:41.063423Z","pmid":"33533916","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}