{"doi":"10.1084/jem.20192080","title":"Layilin augments integrin activation to promote antitumor immunity","abstract":"Tumor-infiltrating CD8+ T cells mediate antitumor immune responses. However, the mechanisms by which T cells remain poised to kill cancer cells despite expressing high levels of inhibitory receptors are unknown. Here, we report that layilin, a C-type lectin domain-containing membrane glycoprotein, is selectively expressed on highly activated, clonally expanded, but phenotypically exhausted CD8+ T cells in human melanoma. Lineage-specific deletion of layilin on murine CD8+ T cells reduced their accumulation in tumors and increased tumor growth in vivo. Congruently, gene editing of LAYN in human CD8+ T cells reduced direct tumor cell killing ex vivo. On a molecular level, layilin colocalized with integrin αLβ2 (LFA-1) on T cells, and cross-linking layilin promoted the activated state of this integrin. Accordingly, LAYN deletion resulted in attenuated LFA-1-dependent cellular adhesion. Collectively, our results identify layilin as part of a molecular pathway in which exhausted or \"dysfunctional\" CD8+ T cells enhance cellular adhesiveness to maintain their cytotoxic potential.","journal":"The Journal of Experimental Medicine","year":2020,"id":59047,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":69,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":310599,"name":"J. Moreau","orcid":"0000-0002-1227-1508","position":1,"is_corresponding":false},{"id":237508,"name":"Jeff E. Glasgow","orcid":"0000-0001-6699-8983","position":2,"is_corresponding":false},{"id":310600,"name":"Devi P Boda","orcid":"0000-0002-2880-1657","position":3,"is_corresponding":false},{"id":258594,"name":"Mariela Pauli","orcid":"0000-0002-9180-4789","position":4,"is_corresponding":false},{"id":310601,"name":"Victoire Gouirand","orcid":"0000-0002-2666-0061","position":5,"is_corresponding":false},{"id":312790,"name":"Luv Panjabi","orcid":null,"position":6,"is_corresponding":false},{"id":312791,"name":"Robby Grewal","orcid":null,"position":7,"is_corresponding":false},{"id":267962,"name":"Jacob M. Luber","orcid":"0000-0001-5268-5732","position":8,"is_corresponding":false},{"id":312792,"name":"Anubhav Mathur","orcid":null,"position":9,"is_corresponding":false},{"id":312793,"name":"R. M. Renny Feldman","orcid":null,"position":10,"is_corresponding":false},{"id":240952,"name":"Eric Shifrut","orcid":"0000-0001-6827-0128","position":11,"is_corresponding":false},{"id":247745,"name":"Pooja Mehta","orcid":"0000-0001-8466-7424","position":12,"is_corresponding":false},{"id":249272,"name":"Margaret M. Lowe","orcid":"0000-0002-5623-7707","position":13,"is_corresponding":false},{"id":310602,"name":"Michael Alvarado","orcid":"0009-0003-7309-567X","position":14,"is_corresponding":false},{"id":30775,"name":"Alexander Marson","orcid":"0000-0002-2734-5776","position":15,"is_corresponding":false},{"id":34472,"name":"Meromit Singer","orcid":"0000-0002-1755-3598","position":16,"is_corresponding":false},{"id":312794,"name":"Jim Wells","orcid":null,"position":17,"is_corresponding":false},{"id":312795,"name":"Ray Jupp","orcid":null,"position":18,"is_corresponding":false},{"id":235283,"name":"Adil Daud","orcid":"0000-0002-6617-8421","position":19,"is_corresponding":false},{"id":254162,"name":"Michael D. Rosenblum","orcid":"0000-0002-0462-5732","position":20,"is_corresponding":false},{"id":267961,"name":"Kelly M. Mahuron","orcid":"0000-0002-2544-5191","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-18T21:07:59.189921Z","pmid":"32539073","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}