{"doi":"10.1084/jem.152.6.1709","title":"Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.","abstract":"<jats:p>To isolate a stable tumor cell line capable of producing human interleukin 2 (IL-2; formerly referred to as T cell growth factor), 16 human T and B leukemia cell lines were screened for constitutive and mitogen-stimulated IL-2 production. We found that the T cell leukemia line designated Jurkat-FHCRC produced &amp;gt; 200 U/ml of IL-2 activity after a 24-h stimulation with T cell mitogens. Peak mitogen-induced IL-2 activity was found in supernates harvested from 24-h Jurkat-FHCRC cell cultures stimulated with either 1% phytohemagglutinin or 20 microgram/ml concanavalin A. Addition of the fatty acid derivative phorbol myristate acetate to mitogen-stimulated cultures increased Jurkat-FHCRC IL-2 production to concentrations &amp;gt; 400 U/ml. IL-2 activity observed in such cases represented between 100--300 times that produced in conventional cultures of mitogen- or alloantigen-stimulated normal human peripheral blood or splenic lymphocytes. Jurkat-FHCRC-derived conditioned medium demonstrated equal capacity to promote the sustained in vitro proliferation of either murine or human activated T cell lines confirming the ability of Jurkat-FHCRC cells to produce human IL-2. These studies identify a new source of human IL-2 and establish a valuable reagent for the isolation and further molecular characterization of this immunoregulatory molecule.</jats:p>","journal":"The Journal of experimental medicine","year":1980,"id":654802,"datarank":0.9140354655067705,"base_score":6.093569770045136,"endowment":6.093569770045136,"self_citation_contribution":0.9140354655067705,"citation_network_contribution":0.0,"self_endowment_contribution":0.9140354655067705,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":442,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1708992,"name":"J Watson","orcid":null,"position":1,"is_corresponding":false},{"id":1708991,"name":"S Gillis","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.","abstract":"<jats:p>To isolate a stable tumor cell line capable of producing human interleukin 2 (IL-2; formerly referred to as T cell growth factor), 16 human T and B leukemia cell lines were screened for constitutive and mitogen-stimulated IL-2 production. We found that the T cell leukemia line designated Jurkat-FHCRC produced &amp;gt; 200 U/ml of IL-2 activity after a 24-h stimulation with T cell mitogens. Peak mitogen-induced IL-2 activity was found in supernates harvested from 24-h Jurkat-FHCRC cell cultures stimulated with either 1% phytohemagglutinin or 20 microgram/ml concanavalin A. Addition of the fatty acid derivative phorbol myristate acetate to mitogen-stimulated cultures increased Jurkat-FHCRC IL-2 production to concentrations &amp;gt; 400 U/ml. IL-2 activity observed in such cases represented between 100--300 times that produced in conventional cultures of mitogen- or alloantigen-stimulated normal human peripheral blood or splenic lymphocytes. Jurkat-FHCRC-derived conditioned medium demonstrated equal capacity to promote the sustained in vitro proliferation of either murine or human activated T cell lines confirming the ability of Jurkat-FHCRC cells to produce human IL-2. These studies identify a new source of human IL-2 and establish a valuable reagent for the isolation and further molecular characterization of this immunoregulatory molecule.</jats:p>","is_dataset_classified":null,"base_score":6.093569770045136,"endowment":6.093569770045136,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6778951","pmcid":null,"openalex_id":"https://openalex.org/W2143167013","authors":[],"funders":[],"total_grants":0,"fwci":12.5107,"citation_percentile":0.99269446,"influential_citations":0,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":6},{"year":2014,"count":5},{"year":2015,"count":5},{"year":2016,"count":4},{"year":2017,"count":6},{"year":2018,"count":4},{"year":2019,"count":4},{"year":2020,"count":4},{"year":2021,"count":4},{"year":2022,"count":3},{"year":2023,"count":6},{"year":2024,"count":4},{"year":2025,"count":6},{"year":2026,"count":4}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jem/article-pdf/152/6/1709/1091380/1709.pdf","host_type":"journal"},{"url":"https://rupress.org/jem/article-pdf/152/6/1709/1091380/1709.pdf","host_type":"publisher"},{"url":"https://rupress.org/jem/article-pdf/152/6/1709/1661603/1709.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1084/jem.152.6.1709","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6778951","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2186024","host_type":"repository"}],"fields_of_study":["Toxin Mechanisms and Immunotoxins","Viral Infectious Diseases and Gene Expression in Insects","Monoclonal and Polyclonal Antibodies Research"],"mesh_terms":["Animals","B-Lymphocytes","Cell Line","Dose-Response Relationship, Drug","Humans","Interleukin-1","Leukemia, Lymphoid","Mitogens","T-Lymphocytes","Tetradecanoylphorbol Acetate","Protein Biosynthesis","Mice","Rats"],"keywords":["Jurkat cells","Interleukin 2","Cell culture","T cell","Molecular biology","Concanavalin A","Biology","Lymphokine","T-cell leukemia","Leukemia","T lymphocyte","Lymphocyte","Phorbol","Cytokine","Immunology","In vitro","Cell biology","Biochemistry","Immune system","Protein kinase C","Signal transduction","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T08:27:55.294850Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}