{"doi":"10.1083/jcb.99.5.1598","title":"Wheat germ agglutinin blocks the acrosome reaction in Strongylocentrotus purpuratus sperm by binding a 210,000-mol-wt membrane protein.","abstract":"<jats:p>Wheat germ agglutinin (WGA) binds to the entire surface of Strongylocentrotus purpuratus sperm, and inhibits the egg jelly-induced acrosome reaction. The binding was found to be species dependent and was completely inhibited by 5 mM N-acetyl-D-glucosamine. Blockage of the acrosome reaction by WGA was bypassed by a combination of the ionophores A23187 and monensin, although neither ionophore was effective individually. These experiments suggest that WGA blocks both Ca2+ uptake and Na+/H+ exchange in these sperm, which was confirmed by direct measurements of 45Ca2+ uptake and H+ efflux. The target of WGA in S. purpuratus sperm appears to be a membrane glycoprotein of Mr = 210,000. Treatment of this protein with neuraminidase or endo-beta-N-acetylglucosaminidase F abolished WGA binding.</jats:p>","journal":"The Journal of cell biology","year":1984,"id":29796,"datarank":3.0084912435620126,"base_score":3.8918202981106265,"endowment":3.8918202981106265,"self_citation_contribution":0.5837730447165941,"citation_network_contribution":2.4247181988454187,"self_endowment_contribution":0.5837730447165941,"citer_contribution":2.4247181988454187,"corpus_percentile":null,"corpus_rank":null,"citation_count":48,"citer_count":35,"citers_with_citation_signal":35,"citers_with_endowment":35,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":163429,"name":"V D Vacquier","orcid":null,"position":1,"is_corresponding":false},{"id":163428,"name":"S B Podell","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.8918202981106265,"endowment":3.8918202981106265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6092388","pmcid":"PMC2113364","openalex_id":"https://openalex.org/W2025403518","authors":[],"funders":[{"funder_name":"NICHD NIH HHS","grant_id":"HD-12896","title":null}],"total_grants":1,"fwci":7.1619,"citation_percentile":0.9645045,"influential_citations":1,"citation_trend":[],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/99/5/1598/1077827/1598.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/99/5/1598/1077827/1598.pdf","host_type":"BRONZE"},{"url":"https://rupress.org/jcb/article-pdf/99/5/1598/1077827/1598.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/99/5/1598/1643310/1598.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.99.5.1598","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6092388","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113364","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2113364","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2113364?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Sperm and Testicular Function","Proteins in Food Systems","Plant Reproductive Biology","Medicine","Biology","Acetylglucosamine","Acrosome","Animals","Calcimycin","Calcium","Lectins","Male","Membrane Proteins","Molecular Weight","Monensin","Neuraminidase","Protons","Receptors, Mitogen","Sea Urchins","Sodium","Species Specificity","Spermatozoa","Wheat Germ Agglutinins"],"mesh_terms":["Calcimycin","Acetylglucosamine","Acrosome","Animals","Calcium","Male","Membrane Proteins","Molecular Weight","Monensin","Neuraminidase","Protons","Receptors, Mitogen","Sea Urchins","Sodium","Species Specificity","Spermatozoa","Wheat Germ Agglutinins","Lectins"],"keywords":["Strongylocentrotus purpuratus","Wheat germ agglutinin","Biology","Acrosome reaction","Sperm","Ionophore","Agglutinin","Acrosome","Sea urchin","Glycoprotein","Biochemistry","Cell biology","Biophysics","Lectin","Membrane","Botany"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T00:46:30.156412Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}