{"doi":"10.1083/jcb.202501219","title":"Kinesin-8/Kip3 requires beta tubulin tail for depolymerase activity","abstract":"Carboxy-terminal tails (CTTs) of tubulin proteins are sites of regulating microtubule function. We previously conducted a genetic interaction screen and identified Kip3, a kinesin-8 motor, as potentially requiring the β-tubulin CTT (β-CTT) for function. Here we use budding yeast to define how β-CTT promotes Kip3 function and the features of β-CTT that are important for this mechanism. We find that β-CTT is necessary for Kip3 depolymerase activity but not for microtubule binding and motility. Mutant yeast cells lacking β-CTT show an increased accumulation of Kip3 at plus ends and along microtubules, but no increase in catastrophe when Kip3 is overexpressed. In vitro experiments show that the β-CTT is necessary for Kip3 to form a tight complex with soluble tubulin but is unnecessary for Kip3 to bind tubulin in the microtubule lattice. These results suggest a model in which β-CTT promotes Kip3 depolymerase activity by supporting a Kip3-tubulin-binding state that is only accessible at the microtubule plus end or in solution.","journal":"The Journal of Cell Biology","year":2025,"id":574393,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.956,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":493854,"name":"Samantha Johnson","orcid":"0000-0001-8963-7881","position":1,"is_corresponding":false},{"id":286055,"name":"Jeffrey K. Moore","orcid":"0000-0003-3262-3248","position":2,"is_corresponding":false},{"id":1481782,"name":"Kaitlin Alemany","orcid":"0000-0002-2594-3203","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T02:57:40.686992Z","pmid":"40920139","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}