{"doi":"10.1083/jcb.202412118","title":"A collagen IV fluorophore knock-in toolkit reveals trimer diversity in <i>C. elegans</i> basement membranes","abstract":"The type IV collagen triple helix, composed of three ⍺-chains, is a core basement membrane (BM) component that assembles into a network within BMs. Endogenous tagging of all ⍺-chains with genetically encoded fluorophores has remained elusive, limiting our understanding of this crucial BM component. Through genome editing, we show that the C termini of the C. elegans type IV collagen ⍺-chains EMB-9 and LET-2 can be fused to a variety of fluorophores to create a strain toolkit with wild-type health. Using quantitative imaging, our results suggest a preference for LET-2-LET-2-EMB-9 trimer construction, but also tissue-specific flexibility in trimers assembled driven by differences in ⍺-chain expression levels. By tagging emb-9 and let-2 mutants that model human Gould syndrome, a complex multitissue disorder, we further discover defects in extracellular accumulation and turnover that might help explain disease pathology. Together, our findings identify a permissive tagging site in C. elegans that will allow diverse studies on type IV collagen regulation and function in animals.","journal":"The Journal of Cell Biology","year":2025,"id":515730,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.958,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":893092,"name":"William Ramos-Lewis","orcid":"0000-0002-4627-4571","position":1,"is_corresponding":false},{"id":810430,"name":"Mychel Morais","orcid":"0000-0001-5237-9524","position":2,"is_corresponding":false},{"id":247640,"name":"Qiuyi Chi","orcid":"0000-0003-1297-4163","position":3,"is_corresponding":false},{"id":686259,"name":"Adam W. J. Soh","orcid":"0000-0003-3931-1765","position":4,"is_corresponding":false},{"id":218186,"name":"E. G. Williams","orcid":"0000-0003-3913-5095","position":5,"is_corresponding":false},{"id":329062,"name":"Rachel Lennon","orcid":"0000-0001-6400-0227","position":6,"is_corresponding":false},{"id":247644,"name":"David R. Sherwood","orcid":"0000-0002-4448-6917","position":7,"is_corresponding":false},{"id":323229,"name":"Sandhya Srinivasan","orcid":"0000-0002-0455-6239","position":0,"is_corresponding":true}],"reference_count":127,"raw_metadata":null,"created_at":"2026-07-19T02:48:44.647022Z","pmid":"40100062","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}