{"doi":"10.1083/jcb.202411107","title":"PCMD-1 stabilizes the PCM scaffold and facilitates centriole separation","abstract":"Centrosomes are highly dynamic organelles, and maintaining their stability is crucial for spindle pole integrity and bipolar spindle formation. Centrosomes consist of a pair of centrioles surrounded by the PCM. In Caenorhabditis elegans, interactions between the scaffold protein SPD-5 and kinase PLK-1 are essential for PCM formation. However, how PCM stability is established and maintained remains unclear. We address this by analyzing the function of PCMD-1, a protein mainly localizing to centrioles. We show that CDK-1 primes PCMD-1 for PLK-1 phosphorylation. Mutations in PLK-1 docking sites abolish PCMD-1 phosphorylation and SPD-5 binding in vitro and destabilize the PCM scaffold in vivo. As a result, microtubule-pulling forces cannot be relayed to centrioles, delaying their separation. Our findings reveal that PCMD-1 is critical for PCM stability and timely centriole separation during PCM disassembly. We propose that PCMD-1 initiates scaffold assembly by biasing the PCM core toward intrinsic order, acting as a seed that propagates throughout the scaffold to ensure structural integrity.","journal":"The Journal of Cell Biology","year":2025,"id":535253,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.951,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1418838,"name":"Astrid Heim","orcid":"0009-0003-2782-3285","position":1,"is_corresponding":false},{"id":1419363,"name":"Luisa Pletschacher","orcid":null,"position":2,"is_corresponding":false},{"id":1418839,"name":"Lisa-Marie Alznauer","orcid":"0009-0005-2765-6565","position":3,"is_corresponding":false},{"id":691032,"name":"Serena Schwenkert","orcid":"0000-0003-4301-5176","position":4,"is_corresponding":false},{"id":1418840,"name":"Friedrich Wolff","orcid":"0009-0003-5753-8469","position":5,"is_corresponding":false},{"id":201938,"name":"Esther Zanin","orcid":"0000-0003-1450-8684","position":6,"is_corresponding":false},{"id":201936,"name":"Tamara Mikeladze-Dvali","orcid":"0000-0002-9449-3218","position":7,"is_corresponding":false},{"id":761181,"name":"Alina Schreiner","orcid":"0009-0004-9772-7528","position":0,"is_corresponding":true}],"reference_count":92,"raw_metadata":null,"created_at":"2026-07-19T02:51:56.297114Z","pmid":"41171095","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}