{"doi":"10.1083/jcb.202404103","title":"A function of TPL/TBL1-type corepressors is to nucleate the assembly of the preinitiation complex","abstract":"The plant corepressor TPL is recruited to diverse chromatin contexts, yet its mechanism of repression remains unclear. Previously, we leveraged the fact that TPL retains its function in a synthetic transcriptional circuit in the yeast model Saccharomyces cerevisiae to localize repressive function to two distinct domains. Here, we employed two unbiased whole-genome approaches to map the physical and genetic interactions of TPL at a repressed locus. We identified SPT4, SPT5, and SPT6 as necessary for repression with SPT4 acting as a bridge connecting TPL to SPT5 and SPT6. We discovered the association of multiple additional constituents of the transcriptional preinitiation complex at TPL-repressed promoters, specifically those involved early in transcription initiation. These findings were validated in yeast and plants, including a novel method to analyze the conditional loss of function of essential genes in plants. Our findings support a model where TPL nucleates preassembly of the transcription activation machinery to facilitate the rapid onset of transcription once repression is relieved.","journal":"The Journal of Cell Biology","year":2024,"id":449707,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9494,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1269525,"name":"Benjamin Downing","orcid":"0000-0002-6654-8947","position":1,"is_corresponding":false},{"id":1269526,"name":"Janet Solano Sanchez","orcid":"0009-0009-2125-2550","position":2,"is_corresponding":false},{"id":719094,"name":"Raphaël Loll‐Krippleber","orcid":"0000-0002-7845-178X","position":3,"is_corresponding":false},{"id":300819,"name":"Nathan M. Belliveau","orcid":"0000-0002-1536-1963","position":4,"is_corresponding":false},{"id":557257,"name":"Ricard A. Rodríguez‐Mias","orcid":"0000-0001-7960-6387","position":5,"is_corresponding":false},{"id":399889,"name":"Andrew J. Bauer","orcid":"0000-0002-3952-881X","position":6,"is_corresponding":false},{"id":1269989,"name":"Isabella J. Watson","orcid":null,"position":7,"is_corresponding":false},{"id":1269990,"name":"Lena Bae","orcid":null,"position":8,"is_corresponding":false},{"id":257491,"name":"Judit Villén","orcid":"0000-0002-1005-1739","position":9,"is_corresponding":false},{"id":225587,"name":"Grant W. Brown","orcid":"0000-0002-9002-5003","position":10,"is_corresponding":false},{"id":121265,"name":"Jennifer L. Nemhauser","orcid":"0000-0002-8909-735X","position":11,"is_corresponding":false},{"id":649212,"name":"Alexander R. Leydon","orcid":"0000-0003-3034-1482","position":0,"is_corresponding":true}],"reference_count":116,"raw_metadata":null,"created_at":"2026-07-19T02:02:20.585759Z","pmid":"39652081","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}