{"doi":"10.1083/jcb.202309128","title":"Dynamic interaction of spliceosomal snRNPs with coilin explains Cajal body characteristics","abstract":"The Cajal body (CB) is a conserved non-membrane nuclear structure where several steps of small nuclear RNP particle (snRNP) biogenesis take place. It has been proposed that CB formation follows a liquid-liquid phase separation model, but this hypothesis has never been rigorously tested. Here, we applied live-cell imaging to show that the key CB assembly factor coilin is mobile within the CB, and we revealed a diffusion barrier that limits the coilin exchange between CBs and the nucleoplasm. We generated single aa mutations and demonstrated that RNA-dependent coilin oligomerization and coilin interaction with snRNP are essential for CB formation and maintenance. We applied these data to formulate a mathematical model that links the movement of coilin within the nucleoplasm, CB, and across the boundary with its oligomerization and snRNP binding. Our results illustrate CB as a structure dynamically responding to snRNP assembly and recycling.","journal":"The Journal of Cell Biology","year":2025,"id":541581,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9596,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1429867,"name":"Michaela Blažíková","orcid":"0000-0003-1227-0393","position":1,"is_corresponding":false},{"id":1060857,"name":"Adriana Roithová","orcid":"0000-0003-0230-4393","position":2,"is_corresponding":false},{"id":1429868,"name":"Martina Hálová","orcid":"0000-0003-0040-9773","position":3,"is_corresponding":false},{"id":1060858,"name":"Nenad Radivojević","orcid":"0000-0001-9484-9002","position":4,"is_corresponding":false},{"id":317998,"name":"Karla M. Neugebauer","orcid":"0000-0002-3835-6761","position":5,"is_corresponding":false},{"id":57109,"name":"David Stanek","orcid":"0000-0002-5865-175X","position":6,"is_corresponding":false},{"id":941804,"name":"Davide A. Basello","orcid":null,"position":0,"is_corresponding":true}],"reference_count":77,"raw_metadata":null,"created_at":"2026-07-19T02:52:47.161928Z","pmid":"40560171","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}