{"doi":"10.1083/jcb.202306048","title":"Genome-scale requirements for dynein-based transport revealed by a high-content arrayed CRISPR screen","abstract":"The microtubule motor dynein plays a key role in cellular organization. However, little is known about how dynein's biosynthesis, assembly, and functional diversity are orchestrated. To address this issue, we have conducted an arrayed CRISPR loss-of-function screen in human cells using the distribution of dynein-tethered peroxisomes and early endosomes as readouts. From a genome-wide gRNA library, 195 validated hits were recovered and parsed into those impacting multiple dynein cargoes and those whose effects are restricted to a subset of cargoes. Clustering of high-dimensional phenotypic fingerprints revealed co-functional proteins involved in many cellular processes, including several candidate novel regulators of core dynein functions. Further analysis of one of these factors, the RNA-binding protein SUGP1, indicates that it promotes cargo trafficking by sustaining functional expression of the dynein activator LIS1. Our data represent a rich source of new hypotheses for investigating microtubule-based transport, as well as several other aspects of cellular organization captured by our high-content imaging.","journal":"The Journal of Cell Biology","year":2024,"id":463164,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9435,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1162286,"name":"Steven Wingett","orcid":"0000-0002-2343-0711","position":1,"is_corresponding":false},{"id":1162287,"name":"Qian Chen","orcid":"0000-0003-2663-7694","position":2,"is_corresponding":false},{"id":1293386,"name":"Morag R. Hunter","orcid":"0000-0003-1226-9923","position":3,"is_corresponding":false},{"id":262478,"name":"J. Matthew Taliaferro","orcid":"0000-0001-7580-1433","position":4,"is_corresponding":false},{"id":1162288,"name":"Douglas Ross‐Thriepland","orcid":"0000-0001-6226-2223","position":5,"is_corresponding":false},{"id":1162289,"name":"Simon L. Bullock","orcid":"0000-0001-9491-4548","position":6,"is_corresponding":false},{"id":1162285,"name":"Chun Hao Wong","orcid":"0000-0001-7614-8616","position":0,"is_corresponding":true}],"reference_count":93,"raw_metadata":null,"created_at":"2026-07-19T02:04:28.838123Z","pmid":"38448164","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}