{"doi":"10.1083/jcb.202203016","title":"Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells","abstract":"Septins are cytoskeletal proteins conserved from algae and protists to mammals. A unique feature of septins is their presence as heteromeric complexes that polymerize into filaments in solution and on lipid membranes. Although animal septins associate extensively with actin-based structures in cells, whether septins organize as filaments in cells and if septin organization impacts septin function is not known. Customizing a tripartite split-GFP complementation assay, we show that all septins decorating actin stress fibers are octamer-containing filaments. Depleting octamers or preventing septins from polymerizing leads to a loss of stress fibers and reduced cell stiffness. Super-resolution microscopy revealed septin fibers with widths compatible with their organization as paired septin filaments. Nanometer-resolved distance measurements and single-protein tracking further showed that septin filaments are membrane bound and largely immobilized. Finally, reconstitution assays showed that septin filaments mediate actin-membrane anchoring. We propose that septin organization as octamer-based filaments is essential for septin function in anchoring and stabilizing actin filaments at the plasma membrane.","journal":"The Journal of Cell Biology","year":2022,"id":237589,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":60,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.95,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":860294,"name":"Cyntia Taveneau","orcid":"0000-0002-3395-4957","position":1,"is_corresponding":false},{"id":860295,"name":"Gerard Castro-Linares","orcid":"0000-0002-7429-3825","position":2,"is_corresponding":false},{"id":860296,"name":"Mikhail Baibakov","orcid":"0000-0002-6969-865X","position":3,"is_corresponding":false},{"id":860297,"name":"Nicolas Buzhinsky","orcid":"0000-0003-2488-5707","position":4,"is_corresponding":false},{"id":860298,"name":"Mar Eroles","orcid":"0000-0003-3571-0769","position":5,"is_corresponding":false},{"id":860299,"name":"Violeta Milanović","orcid":"0000-0001-6319-6969","position":6,"is_corresponding":false},{"id":459234,"name":"Shizue Omi","orcid":"0000-0002-2711-2016","position":7,"is_corresponding":false},{"id":860300,"name":"J.D. 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