{"doi":"10.1083/jcb.202105067","title":"Label-retention expansion microscopy","abstract":"Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.","journal":"The Journal of Cell Biology","year":2021,"id":150990,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":87,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.955,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":272016,"name":"Qi Li","orcid":"0000-0002-6891-5923","position":1,"is_corresponding":false},{"id":564555,"name":"Zhipeng Dai","orcid":"0000-0003-4716-3096","position":2,"is_corresponding":false},{"id":273173,"name":"Arthur A. Tran","orcid":null,"position":3,"is_corresponding":false},{"id":495633,"name":"Siyu Feng","orcid":"0000-0001-8499-6914","position":4,"is_corresponding":false},{"id":624153,"name":"Alejandro Ramirez","orcid":"0000-0002-8803-8630","position":5,"is_corresponding":false},{"id":641925,"name":"Zixi Lin","orcid":"0009-0003-4710-3963","position":6,"is_corresponding":false},{"id":641926,"name":"Xiaomeng Wang","orcid":"0000-0003-2169-3013","position":7,"is_corresponding":false},{"id":473881,"name":"Tracy T. Chow","orcid":"0000-0002-1690-8035","position":8,"is_corresponding":false},{"id":331766,"name":"Jiapei Chen","orcid":"0000-0002-8205-8593","position":9,"is_corresponding":false},{"id":288713,"name":"Dhivya Kumar","orcid":"0000-0002-3737-014X","position":10,"is_corresponding":false},{"id":641927,"name":"Andrew McColloch","orcid":"0000-0003-3256-5826","position":11,"is_corresponding":false},{"id":74141,"name":"Jeremy F. Reiter","orcid":"0000-0002-6512-320X","position":12,"is_corresponding":false},{"id":226820,"name":"Eric J. Huang","orcid":"0000-0002-5381-3801","position":13,"is_corresponding":false},{"id":272028,"name":"Ian B. Seiple","orcid":"0000-0002-8732-1362","position":14,"is_corresponding":false},{"id":406504,"name":"Bo Huang","orcid":"0000-0003-1704-4141","position":15,"is_corresponding":false},{"id":564556,"name":"Xiaoyu Shi","orcid":"0000-0002-9634-2659","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-18T23:43:11.293086Z","pmid":"34228783","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}