{"doi":"10.1083/jcb.202009083","title":"Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome","abstract":"Centrosomes are composed of a centriolar core surrounded by a pericentriolar material (PCM) matrix that docks microtubule-nucleating γ-tubulin complexes. During mitotic entry, the PCM matrix increases in size and nucleating capacity in a process called centrosome maturation. Polo-like kinase 1 (PLK1) is recruited to centrosomes and phosphorylates PCM matrix proteins to drive their self-assembly, which leads to PCM expansion. Here, we show that in addition to controlling PCM expansion, PLK1 independently controls the generation of binding sites for γ-tubulin complexes on the PCM matrix. Selectively preventing the generation of PLK1-dependent γ-tubulin docking sites led to spindle defects and impaired chromosome segregation without affecting PCM expansion, highlighting the importance of phospho-regulated centrosomal γ-tubulin docking sites in spindle assembly. Inhibiting both γ-tubulin docking and PCM expansion by mutating substrate target sites recapitulated the effects of loss of centrosomal PLK1 on the ability of centrosomes to catalyze spindle assembly.","journal":"The Journal of Cell Biology","year":2020,"id":56641,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":84,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9474,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":294677,"name":"Zhiling Zhao","orcid":"0000-0003-0623-5057","position":1,"is_corresponding":false},{"id":294678,"name":"Di Wu","orcid":"0000-0002-6241-2102","position":2,"is_corresponding":false},{"id":288895,"name":"Shaohe Wang","orcid":"0000-0001-9522-2359","position":3,"is_corresponding":false},{"id":294679,"name":"Jennifer L Harrison","orcid":"0000-0002-7125-4740","position":4,"is_corresponding":false},{"id":204250,"name":"J. Sebastián Gómez-Cavazos","orcid":null,"position":5,"is_corresponding":false},{"id":256454,"name":"Arshad Desai","orcid":"0000-0002-5410-1830","position":6,"is_corresponding":false},{"id":256455,"name":"Karen Oegema","orcid":"0000-0001-8515-7514","position":7,"is_corresponding":false},{"id":256450,"name":"Midori Ohta","orcid":"0000-0001-8189-6846","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-18T21:06:09.605575Z","pmid":"33399854","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}