{"doi":"10.1083/jcb.201908164","title":"Citrullination regulates wound responses and tissue regeneration in zebrafish","abstract":"Calcium is an important early signal in wound healing, yet how these early signals promote regeneration remains unclear. Peptidylarginine deiminases (PADs), a family of calcium-dependent enzymes, catalyze citrullination, a post-translational modification that alters protein function and has been implicated in autoimmune diseases. We generated a mutation in the single zebrafish ancestral pad gene, padi2, that results in a loss of detectable calcium-dependent citrullination. The mutants exhibit impaired resolution of inflammation and regeneration after caudal fin transection. We identified a new subpopulation of cells displaying citrullinated histones within the notochord bead following tissue injury. Citrullination of histones in this region was absent, and wound-induced proliferation was perturbed in Padi2-deficient larvae. Taken together, our results show that Padi2 is required for the citrullination of histones within a group of cells in the notochord bead and for promoting wound-induced proliferation required for efficient regeneration. These findings identify Padi2 as a potential intermediary between early calcium signaling and subsequent tissue regeneration.","journal":"The Journal of Cell Biology","year":2020,"id":64805,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9539,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":342386,"name":"Jayne M. Squirrell","orcid":"0000-0003-0651-6027","position":1,"is_corresponding":false},{"id":342387,"name":"David A. Bennin","orcid":"0000-0001-6137-8419","position":2,"is_corresponding":false},{"id":342388,"name":"Julie Rindy","orcid":"0000-0002-4242-6427","position":3,"is_corresponding":false},{"id":342389,"name":"Paige E. Pistono","orcid":"0000-0003-2517-4288","position":4,"is_corresponding":false},{"id":72584,"name":"Kevin W. Eliceiri","orcid":"0000-0001-8678-670X","position":5,"is_corresponding":false},{"id":342390,"name":"Miriam A. Shelef","orcid":"0000-0002-8842-0303","position":6,"is_corresponding":false},{"id":116929,"name":"Junsu Kang","orcid":"0000-0001-5286-5426","position":7,"is_corresponding":false},{"id":342391,"name":"Anna Huttenlocher","orcid":"0000-0001-7940-6254","position":8,"is_corresponding":false},{"id":343452,"name":"Netta Golenberg","orcid":null,"position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-18T21:12:58.081635Z","pmid":"32328635","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}