{"doi":"10.1083/jcb.201702135","title":"COPII-coated membranes function as transport carriers of intracellular procollagen I","abstract":"<jats:p>The coat protein complex II (COPII) is essential for the transport of large cargo, such as 300-nm procollagen I (PC1) molecules, from the endoplasmic reticulum (ER) to the Golgi. Previous work has shown that the CUL3-KLHL12 complex increases the size of COPII vesicles at ER exit sites to more than 300 nm in diameter and accelerates the secretion of PC1. However, the role of large COPII vesicles as PC1 transport carriers was not unambiguously demonstrated. In this study, using stochastic optical reconstruction microscopy, correlated light electron microscopy, and live-cell imaging, we demonstrate the existence of mobile COPII-coated vesicles that completely encapsulate the cargo PC1 and are physically separated from ER. We also developed a cell-free COPII vesicle budding reaction that reconstitutes the capture of PC1 into large COPII vesicles. This process requires COPII proteins and the GTPase activity of the COPII subunit SAR1. We conclude that large COPII vesicles are bona fide carriers of PC1.</jats:p>","journal":"Journal of Cell Biology","year":2017,"id":18328,"datarank":3.755027816822592,"base_score":4.718498871295094,"endowment":4.718498871295094,"self_citation_contribution":0.7077748306942643,"citation_network_contribution":3.0472529861283277,"self_endowment_contribution":0.7077748306942643,"citer_contribution":3.0472529861283277,"corpus_percentile":null,"corpus_rank":null,"citation_count":111,"citer_count":99,"citers_with_citation_signal":88,"citers_with_endowment":88,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":56852,"name":"Lin Yuan","orcid":"0000-0001-5556-0994","position":1,"is_corresponding":false},{"id":127788,"name":"Samuel J. 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Transducing","Multivesicular Bodies"],"keywords":["COPII","COPI","Vesicle","Endoplasmic reticulum","Cell biology","Golgi apparatus","Vesicular Transport Proteins","Secretion","Biophysics","Membrane","Biology","Intracellular","Secretory pathway","Biochemistry","Time Factors","1.1 Normal biological development and functioning","Transfection","Medical and Health Sciences","Coatomer Protein","Fluorescence","Collagen Type I","Cell Line","Rare Diseases","Underpinning research","Cell Line, Tumor","Humans","Microscopy, Immunoelectron","alpha 1 Chain","Research Articles","Adaptor Proteins, Signal Transducing","Monomeric GTP-Binding Proteins","Immunoelectron","Microscopy","Tumor","Microscopy, Video","Microfilament Proteins","Signal Transducing","Multivesicular Bodies","Adaptor Proteins","Video","Biological Sciences","Cullin Proteins","Collagen Type I, alpha 1 Chain","Protein Transport","Microscopy, Fluorescence","Generic health relevance","COP-Coated Vesicles","Procollagen","Developmental 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