{"doi":"10.1083/jcb.201503075","title":"NBR1 enables autophagy-dependent focal adhesion turnover","abstract":"<jats:p>Autophagy is a catabolic pathway involving the sequestration of cellular contents into a double-membrane vesicle, the autophagosome. Although recent studies have demonstrated that autophagy supports cell migration, the underlying mechanisms remain unknown. Using live-cell imaging, we uncover that autophagy promotes optimal migratory rate and facilitates the dynamic assembly and disassembly of cell-matrix focal adhesions (FAs), which is essential for efficient motility. Additionally, our studies reveal that autophagosomes associate with FAs primarily during disassembly, suggesting autophagy locally facilitates the destabilization of cell-matrix contact sites. Furthermore, we identify the selective autophagy cargo receptor neighbor of BRCA1 (NBR1) as a key mediator of autophagy-dependent FA remodeling. NBR1 depletion impairs FA turnover and decreases targeting of autophagosomes to FAs, whereas ectopic expression of autophagy-competent, but not autophagy-defective, NBR1 enhances FA disassembly and reduces FA lifetime during migration. Our findings provide mechanistic insight into how autophagy promotes migration by revealing a requirement for NBR1-mediated selective autophagy in enabling FA disassembly in motile cells.</jats:p>","journal":"Journal of Cell Biology","year":2016,"id":31159,"datarank":5.8364674912887065,"base_score":5.10594547390058,"endowment":5.10594547390058,"self_citation_contribution":0.7658918210850871,"citation_network_contribution":5.0705756702036195,"self_endowment_contribution":0.7658918210850871,"citer_contribution":5.0705756702036195,"corpus_percentile":null,"corpus_rank":null,"citation_count":164,"citer_count":158,"citers_with_citation_signal":141,"citers_with_endowment":141,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":167605,"name":"Samantha J. 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