{"doi":"10.1083/jcb.201501118","title":"Tracking individual secretory vesicles during exocytosis reveals an ordered and regulated process","abstract":"<jats:p>Post-Golgi secretory vesicle trafficking is a coordinated process, with transport and regulatory mechanisms to ensure appropriate exocytosis. While the contributions of many individual regulatory proteins to this process are well studied, the timing and dependencies of events have not been defined. Here we track individual secretory vesicles and associated proteins in vivo during tethering and fusion in budding yeast. Secretory vesicles tether to the plasma membrane very reproducibly for ∼18 s, which is extended in cells defective for membrane fusion and significantly lengthened and more variable when GTP hydrolysis of the exocytic Rab is delayed. Further, the myosin-V Myo2p regulates the tethering time in a mechanism unrelated to its interaction with exocyst component Sec15p. Two-color imaging of tethered vesicles with Myo2p, the GEF Sec2p, and several exocyst components allowed us to document a timeline for yeast exocytosis in which Myo2p leaves 4 s before fusion, whereas Sec2p and all the components of the exocyst disperse coincident with fusion.</jats:p>","journal":"Journal of Cell Biology","year":2015,"id":37668,"datarank":2.7313132562000986,"base_score":4.3694478524670215,"endowment":4.3694478524670215,"self_citation_contribution":0.6554171778700533,"citation_network_contribution":2.0758960783300453,"self_endowment_contribution":0.6554171778700533,"citer_contribution":2.0758960783300453,"corpus_percentile":null,"corpus_rank":null,"citation_count":78,"citer_count":74,"citers_with_citation_signal":61,"citers_with_endowment":61,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":188293,"name":"Anthony Bretscher","orcid":null,"position":1,"is_corresponding":false},{"id":188292,"name":"Kirk W. Donovan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.3694478524670215,"endowment":4.3694478524670215,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26169352","pmcid":"PMC4508886","openalex_id":"https://openalex.org/W1943574736","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM39066","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM039066","title":null},{"funder_name":"National Institutes of Health","grant_id":"2R01GM039066-22","title":"Microfilaments in the yeast Saccharomyces cerevisiae"}],"total_grants":3,"fwci":4.2383,"citation_percentile":0.94466482,"influential_citations":5,"citation_trend":[{"year":2015,"count":3},{"year":2016,"count":5},{"year":2017,"count":11},{"year":2018,"count":8},{"year":2019,"count":8},{"year":2020,"count":5},{"year":2021,"count":9},{"year":2022,"count":6},{"year":2023,"count":6},{"year":2024,"count":6},{"year":2025,"count":10},{"year":2026,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/210/2/181/672114/jcb_201501118.pdf","host_type":"journal"},{"url":"https://europepmc.org/articles/pmc4508886?pdf=render","host_type":"GREEN"},{"url":"https://rupress.org/jcb/article-pdf/210/2/181/672114/jcb_201501118.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/210/2/181/1598255/jcb_201501118.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.201501118","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26169352","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4508886","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4508886","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4508886?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1083/jcb.201501118","host_type":""},{"url":"https://dx.doi.org/10.1083/jcb.201501118","host_type":""}],"fields_of_study":["Cellular transport and secretion","Fungal and yeast genetics research","Genetic Neurodegenerative Diseases","Medicine","Biology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Membrane Fusion","Microscopy, Fluorescence","Myosin Heavy Chains","Myosin Type V","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Secretory Pathway","Secretory Vesicles","rab GTP-Binding Proteins"],"mesh_terms":["Membrane Fusion","Microscopy, Fluorescence","Saccharomyces cerevisiae","Myosin Heavy Chains","rab GTP-Binding Proteins","Secretory Vesicles","Myosin Type V","Saccharomyces cerevisiae Proteins","Secretory Pathway"],"keywords":["Exocyst","Exocytosis","Biology","Vesicle","Secretory Vesicle","Cell biology","Vesicle fusion","Lipid bilayer fusion","Secretory pathway","Rab","Golgi apparatus","GTPase","Biochemistry","Membrane","Synaptic vesicle","Saccharomyces cerevisiae Proteins","Microscopy, Fluorescence","Myosin Heavy Chains","rab GTP-Binding Proteins","Secretory Vesicles","Myosin Type V","Saccharomyces cerevisiae","Membrane Fusion","Research Articles"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.26709587.v1","title":"Additional file 1 of Small GTPase Rab7 is involved in stress adaptation to carbon starvation to ensure the induced cellulase biosynthesis in Trichoderma reesei","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26709587","title":"Additional file 1 of Small GTPase Rab7 is involved in stress adaptation to carbon starvation to ensure the induced cellulase biosynthesis in Trichoderma reesei","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T20:33:09.478173Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}