{"doi":"10.1083/jcb.201209023","title":"Sanpodo controls sensory organ precursor fate by directing Notch trafficking and binding γ-secretase","abstract":"<jats:p>In Drosophila peripheral neurogenesis, Notch controls cell fates in sensory organ precursor (SOP) cells. SOPs undergo asymmetric cell division by segregating Numb, which inhibits Notch signaling, into the pIIb daughter cell after cytokinesis. In contrast, in the pIIa daughter cell, Notch is activated and requires Sanpodo, but its mechanism of action has not been elucidated. As Sanpodo is present in both pIIa and pIIb cells, a second role for Sanpodo in regulating Notch signaling in the low-Notch pIIb cell has been proposed. Here we demonstrate that Sanpodo regulates Notch signaling levels in both pIIa and pIIb cells via distinct mechanisms. The interaction of Sanpodo with Presenilin, a component of the γ-secretase complex, was required for Notch activation and pIIa cell fate. In contrast, Sanpodo suppresses Notch signaling in the pIIb cell by driving Notch receptor internalization. Together, these results demonstrate that a single protein can regulate Notch signaling through distinct mechanisms to either promote or suppress signaling depending on the local cellular context.</jats:p>","journal":"Journal of Cell Biology","year":2013,"id":597845,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1531676,"name":"Vasundhara Kandachar","orcid":null,"position":1,"is_corresponding":false},{"id":1531677,"name":"Diana Zitserman","orcid":null,"position":2,"is_corresponding":false},{"id":1483907,"name":"Xin Tong","orcid":"0000-0001-6105-5345","position":3,"is_corresponding":false},{"id":1531678,"name":"Fabrice Roegiers","orcid":null,"position":4,"is_corresponding":false},{"id":1531675,"name":"Alok Upadhyay","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Sanpodo controls sensory organ precursor fate by directing Notch trafficking and binding γ-secretase","abstract":"<jats:p>In Drosophila peripheral neurogenesis, Notch controls cell fates in sensory organ precursor (SOP) cells. SOPs undergo asymmetric cell division by segregating Numb, which inhibits Notch signaling, into the pIIb daughter cell after cytokinesis. In contrast, in the pIIa daughter cell, Notch is activated and requires Sanpodo, but its mechanism of action has not been elucidated. As Sanpodo is present in both pIIa and pIIb cells, a second role for Sanpodo in regulating Notch signaling in the low-Notch pIIb cell has been proposed. Here we demonstrate that Sanpodo regulates Notch signaling levels in both pIIa and pIIb cells via distinct mechanisms. The interaction of Sanpodo with Presenilin, a component of the γ-secretase complex, was required for Notch activation and pIIa cell fate. In contrast, Sanpodo suppresses Notch signaling in the pIIb cell by driving Notch receptor internalization. Together, these results demonstrate that a single protein can regulate Notch signaling through distinct mechanisms to either promote or suppress signaling depending on the local cellular context.</jats:p>","is_dataset_classified":null,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23609534","pmcid":"PMC3639393","openalex_id":"https://openalex.org/W2055949004","authors":[],"funders":[{"funder_name":"NINDS NIH HHS","grant_id":"R01NS059971","title":null},{"funder_name":"National Institutes of Health","grant_id":"1R01NS059971-01A2","title":"Endocytic Control of Notch-Mediated Cell Fate Decisions in Neurogenesis"}],"total_grants":2,"fwci":1.18,"citation_percentile":0.78084318,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":3},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":1}],"oa_status":"hybrid","license":"cc-by-nc-sa","oa_locations":[{"url":"http://jcb.rupress.org/content/jcb/201/3/439.full.pdf","host_type":"journal"},{"url":"http://jcb.rupress.org/content/jcb/201/3/439.full.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/201/3/439/1579233/jcb_201209023.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.201209023","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23609534","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3639393","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3639393","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3639393?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1083/jcb.201209023","host_type":""},{"url":"https://dx.doi.org/10.1083/jcb.201209023","host_type":""}],"fields_of_study":["Developmental Biology and Gene Regulation","Marine Invertebrate Physiology and Ecology","Neurobiology and Insect Physiology Research","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Cell Line","Endocytosis","Mechanoreceptors","Microfilament Proteins","Protein Binding","Stem Cells","Amino Acid Motifs","Protein Subunits","Protein Transport","Drosophila Proteins","Receptors, Notch","Presenilins","Amyloid Precursor Protein Secretases","Protein Interaction Domains and Motifs"],"keywords":["Notch signaling pathway","Biology","NUMB","Cell fate determination","Cell biology","Notch proteins","Hes3 signaling axis","Neurogenesis","Signal transduction","Cell signaling","Transcription factor","Genetics","Receptors, Notch","Stem Cells","Amino Acid Motifs","Microfilament Proteins","Presenilins","Endocytosis","Cell Line","Protein Subunits","Protein Transport","Drosophila Proteins","Protein Interaction Domains and Motifs","Amyloid Precursor Protein Secretases","Mechanoreceptors","Research Articles","Protein Binding"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T14:24:42.677417Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}