{"doi":"10.1083/jcb.201004109","title":"Cajal body surveillance of U snRNA export complex assembly","abstract":"<jats:p>Phosphorylated adaptor for RNA export (PHAX) is the key export mediator for spliceosomal U small nuclear RNA (snRNA) precursors in metazoa. PHAX is enriched in Cajal bodies (CBs), nuclear subdomains involved in the biogenesis of small ribonucleoproteins. However, CBs’ role in U snRNA export has not been demonstrated. In this study, we show that U snRNA precursors microinjected into Xenopus laevis oocyte nuclei temporarily concentrate in CBs but gradually decrease as RNA export proceeds. Inhibition of PHAX activity by the coinjection of a specific anti-PHAX antibody or a dominant-negative PHAX mutant inhibits U snRNA export and simultaneously enhances accumulation of U snRNA precursors in CBs, indicating that U snRNAs transit through CBs before export and that binding to PHAX is required for efficient exit of U snRNAs from CBs. Similar results were obtained with U snRNAs transcribed from microinjected genes. These results reveal a novel function for CBs, which ensure that U snRNA precursors are properly bound by PHAX.</jats:p>","journal":"Journal of Cell Biology","year":2010,"id":34519,"datarank":1.738328478749855,"base_score":3.784189633918261,"endowment":3.784189633918261,"self_citation_contribution":0.5676284450877392,"citation_network_contribution":1.1707000336621156,"self_endowment_contribution":0.5676284450877392,"citer_contribution":1.1707000336621156,"corpus_percentile":null,"corpus_rank":null,"citation_count":43,"citer_count":41,"citers_with_citation_signal":36,"citers_with_endowment":36,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":132729,"name":"Hiroto Izumi","orcid":null,"position":1,"is_corresponding":false},{"id":178674,"name":"Mutsuhito Ohno","orcid":null,"position":2,"is_corresponding":false},{"id":178673,"name":"Tatsuya Suzuki","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.784189633918261,"endowment":3.784189633918261,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20733056","pmcid":"PMC2928011","openalex_id":"https://openalex.org/W2116932998","authors":[],"funders":[],"total_grants":0,"fwci":1.0888,"citation_percentile":0.76601654,"influential_citations":4,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":2},{"year":2014,"count":6},{"year":2015,"count":5},{"year":2016,"count":6},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":5},{"year":2024,"count":2},{"year":2025,"count":3}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/190/4/603/1348638/jcb_201004109.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/190/4/603/1348638/jcb_201004109.pdf","host_type":"HYBRID"},{"url":"https://rupress.org/jcb/article-pdf/190/4/603/1348638/jcb_201004109.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/190/4/603/1569045/jcb_201004109.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.201004109","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20733056","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2928011","host_type":"repository"},{"url":"http://jcb.rupress.org/content/190/4/603.full.pdf","host_type":"Unpaywall"},{"url":"https://europepmc.org/articles/PMC2928011","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2928011?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["RNA Research and Splicing","RNA modifications and cancer","Nuclear Structure and Function","Biology","Medicine"],"mesh_terms":["Exportin 1 Protein","Amino Acid Sequence","Animals","Cell Nucleus","Molecular Sequence Data","Oocytes","RNA Precursors","RNA, Small Nuclear","Xenopus laevis","Receptors, Cytoplasmic and Nuclear","Coiled Bodies","Active Transport, Cell Nucleus","Karyopherins","Nucleocytoplasmic Transport Proteins"],"keywords":["Small nuclear RNA","snRNP","Cajal body","Biology","Ribonucleoprotein","Cell biology","Prp24","Small nuclear ribonucleoprotein","Biogenesis","RNA","Nuclear export signal","Molecular biology","RNA splicing","Cell nucleus","Biochemistry","Gene","Non-coding RNA","Nucleus"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T18:56:12.569323Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}