{"doi":"10.1083/jcb.200901029","title":"Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia","abstract":"<jats:p>Maintenance and remodeling of adherens junctions (AJs) and cell shape in epithelia are necessary for the development of functional epithelia and are commonly altered during cancer progression/metastasis. Although formation of nascent AJs has received much attention, whether shared mechanisms are responsible for the maintenance and remodeling of AJs in dynamic epithelia, particularly in vivo, is not clear. Using clonal analysis in the postmitotic Drosophila melanogaster pupal eye epithelium, we demonstrate that Rho1 is required to maintain AJ integrity independent of its role in sustaining apical cell tension. Rho1 depletion in a remodeling postmitotic epithelium disrupts AJs but only when depleted in adjacent cells. Surprisingly, neither of the Rho effectors, Rok or Dia, is necessary downstream of Rho1 to maintain AJs; instead, Rho1 maintains AJs by inhibiting Drosophila epithelial cadherin endocytosis in a Cdc42/Par6-dependent manner. In contrast, depletion of Rho1 in single cells decreases apical tension, and Rok and myosin are necessary, while Dia function also contributes, downstream of Rho1 to sustain apical cell tension.</jats:p>","journal":"Journal of Cell Biology","year":2009,"id":43519,"datarank":2.6233588723879535,"base_score":4.189654742026425,"endowment":4.189654742026425,"self_citation_contribution":0.6284482113039639,"citation_network_contribution":1.9949106610839897,"self_endowment_contribution":0.6284482113039639,"citer_contribution":1.9949106610839897,"corpus_percentile":null,"corpus_rank":null,"citation_count":65,"citer_count":57,"citers_with_citation_signal":51,"citers_with_endowment":51,"datacite_reuse_total":4,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":206602,"name":"Gregory D. Longmore","orcid":null,"position":1,"is_corresponding":false},{"id":206601,"name":"Stephen J. Warner","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.189654742026425,"endowment":4.189654742026425,"datacite_reuse_total":4,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19506041","pmcid":"PMC2711606","openalex_id":"https://openalex.org/W2121677205","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM080673","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM080673","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA085839","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA85839","title":null},{"funder_name":"National Institutes of Health","grant_id":"3R01CA085839-03S1","title":"ROLE OF LIM PROTEINS IN REGULATING CELL GROWTH"},{"funder_name":"National Institutes of Health","grant_id":"5R01GM080673-06","title":"EPITHELIAL MORPHOGENESIS IN DEVELOPMENT AND DISEASE"}],"total_grants":6,"fwci":2.8755,"citation_percentile":0.91101633,"influential_citations":5,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":6},{"year":2014,"count":7},{"year":2015,"count":5},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":3},{"year":2020,"count":2},{"year":2021,"count":5},{"year":2022,"count":2},{"year":2023,"count":4},{"year":2024,"count":5}],"oa_status":"hybrid","license":"cc-by-nc-sa","oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/185/6/1111/1342734/jcb_200901029.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/185/6/1111/1342734/jcb_200901029.pdf","host_type":"HYBRID"},{"url":"https://rupress.org/jcb/article-pdf/185/6/1111/1342734/jcb_200901029.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/185/6/1111/1899046/jcb_200901029.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.200901029","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19506041","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2711606","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2711606","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2711606?pdf=render","host_type":"Europe_PMC"},{"url":"http://jcb.rupress.org/content/185/6/1111.full.pdf","host_type":""},{"url":"http://dx.doi.org/10.1083/jcb.200901029","host_type":""},{"url":"https://dx.doi.org/10.1083/jcb.200901029","host_type":""}],"fields_of_study":["Wnt/β-catenin signaling in development and cancer","Cellular transport and secretion","Hippo pathway signaling and YAP/TAZ","Biology","Medicine","0301 basic medicine","0303 health sciences","03 medical and health sciences","Adaptor Proteins, Signal Transducing","Adherens Junctions","Animals","Armadillo Domain Proteins","Cadherins","Carrier Proteins","Cell Membrane","Cell Polarity","Drosophila Proteins","Drosophila melanogaster","Epithelial Cells","Epithelium","Formins","Isoenzymes","Myosins","Photoreceptor Cells, Invertebrate","RNA Interference","Stress, Mechanical","Transcription Factors","cdc42 GTP-Binding Protein","rho GTP-Binding Proteins","rho-Associated Kinases"],"mesh_terms":["Formins","Animals","Carrier Proteins","Cell Membrane","Drosophila melanogaster","Epithelial Cells","Epithelium","Isoenzymes","Myosins","Stress, Mechanical","Transcription Factors","Cadherins","Cell Polarity","Photoreceptor Cells, Invertebrate","rho GTP-Binding Proteins","cdc42 GTP-Binding Protein","Adherens 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