{"doi":"10.1083/jcb.200609088","title":"The budding yeast PP2ACdc55 protein phosphatase prevents the onset of anaphase in response to morphogenetic defects","abstract":"<jats:p>Faithful chromosome transmission requires establishment of sister chromatid cohesion during S phase, followed by its removal at anaphase onset. Sister chromatids are tethered together by cohesin, which is displaced from chromosomes through cleavage of its Mcd1 subunit by the separase protease. Separase is in turn inhibited, up to this moment, by securin. Budding yeast cells respond to morphogenetic defects by a transient arrest in G2 with high securin levels and unseparated chromatids. We show that neither securin elimination nor forced cohesin cleavage is sufficient for anaphase in these conditions, suggesting that other factors contribute to cohesion maintainance in G2. We find that the protein phosphatase PP2A bound to its regulatory subunit Cdc55 plays a key role in this process, uncovering a new function for PP2ACdc55 in controlling a noncanonical pathway of chromatid cohesion removal.</jats:p>","journal":"The Journal of Cell Biology","year":2007,"id":687919,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":912300,"name":"Valentina Rossio","orcid":"0000-0002-7353-8640","position":1,"is_corresponding":false},{"id":1611764,"name":"Giovanna Lucchini","orcid":null,"position":2,"is_corresponding":false},{"id":201979,"name":"Simonetta Piatti","orcid":null,"position":3,"is_corresponding":false},{"id":1384875,"name":"Elena Chiroli","orcid":"0000-0001-8668-0657","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The budding yeast PP2ACdc55 protein phosphatase prevents the onset of anaphase in response to morphogenetic defects","abstract":"<jats:p>Faithful chromosome transmission requires establishment of sister chromatid cohesion during S phase, followed by its removal at anaphase onset. Sister chromatids are tethered together by cohesin, which is displaced from chromosomes through cleavage of its Mcd1 subunit by the separase protease. Separase is in turn inhibited, up to this moment, by securin. Budding yeast cells respond to morphogenetic defects by a transient arrest in G2 with high securin levels and unseparated chromatids. We show that neither securin elimination nor forced cohesin cleavage is sufficient for anaphase in these conditions, suggesting that other factors contribute to cohesion maintainance in G2. We find that the protein phosphatase PP2A bound to its regulatory subunit Cdc55 plays a key role in this process, uncovering a new function for PP2ACdc55 in controlling a noncanonical pathway of chromatid cohesion removal.</jats:p>","is_dataset_classified":null,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17502422","pmcid":null,"openalex_id":"https://openalex.org/W2099184692","authors":[],"funders":[{"funder_name":"Telethon","grant_id":"GGP05034","title":null}],"total_grants":1,"fwci":0.7283,"citation_percentile":0.67991657,"influential_citations":0,"citation_trend":[{"year":2013,"count":4},{"year":2014,"count":1},{"year":2018,"count":1},{"year":2021,"count":2},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2064206","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2064206","host_type":"repository"},{"url":"https://rupress.org/jcb/article-pdf/177/4/599/1879004/jcb_200609088.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.200609088","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17502422","host_type":"repository"},{"url":"http://hdl.handle.net/10281/314","host_type":"repository"}],"fields_of_study":["Microtubule and mitosis dynamics","Photosynthetic Processes and Mechanisms","Fungal and yeast genetics research","Anaphase","Cell Cycle Proteins","Chromatids","Nuclear Proteins","Phosphoprotein Phosphatases","Protein Phosphatase 2","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Securin"],"mesh_terms":["Anaphase","Chromatids","Nuclear Proteins","Phosphoprotein Phosphatases","Saccharomyces cerevisiae","Cell Cycle Proteins","Saccharomyces cerevisiae Proteins","Protein Phosphatase 2","Securin"],"keywords":["Separase","Cohesin","Securin","Anaphase","Establishment of sister chromatid cohesion","Biology","Cell biology","Sister chromatids","Protein phosphatase 2","Chromatid","Kinetochore","Chromosome segregation","Anaphase-promoting complex","Genetics","Cell cycle","Phosphatase","Chromosome"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-19T09:26:31.139016Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}