{"doi":"10.1083/jcb.200406024","title":"Adherens junction-dependent and -independent steps in the establishment of epithelial cell polarity in\n                    <i>Drosophila</i>","abstract":"<jats:p>Adherens junctions (AJs) are thought to be key landmarks for establishing epithelial cell polarity, but the origin of epithelial polarity in Drosophila remains unclear. Thus, we examined epithelial polarity establishment during early Drosophila development. We found apical accumulation of both Drosophila E-Cadherin (DE-Cad) and the apical cue Bazooka (Baz) as cells first form. Mutant analyses revealed that apical Baz accumulations can be established in the absence of AJs, whereas assembly of apical DE-Cad complexes requires Baz. Thus, Baz acts upstream of AJs during epithelial polarity establishment. During gastrulation the absence of AJs results in widespread cell dissociation and depolarization. Some epithelial structures are retained, however. These structures maintain apical Baz, accumulate apical Crumbs, and organize polarized cytoskeletons, but display abnormal cell morphology and fail to segregate the basolateral cue Discs large from the apical domain. Thus, although epithelial polarity develops in the absence of AJs, AJs play specific roles in maintaining epithelial architecture and segregating basolateral cues.</jats:p>","journal":"The Journal of Cell Biology","year":2004,"id":42544,"datarank":9.585734053842003,"base_score":5.60947179518496,"endowment":5.60947179518496,"self_citation_contribution":0.8414207692777441,"citation_network_contribution":8.744313284564258,"self_endowment_contribution":0.8414207692777441,"citer_contribution":8.744313284564258,"corpus_percentile":null,"corpus_rank":null,"citation_count":272,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":203139,"name":"Mark Peifer","orcid":null,"position":1,"is_corresponding":false},{"id":203518,"name":"Tony J.C. Harris","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.60947179518496,"endowment":5.60947179518496,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15479740","pmcid":"PMC2172516","openalex_id":"https://openalex.org/W1968981755","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM 47857","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM047857","title":null},{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"unidentified","title":"unidentified"},{"funder_name":"National Institutes of Health","grant_id":"5R01GM047857-05","title":"ADHESIVE JUNCTIONS AND SIGNAL TRANSDUCTION IN DROSOPHILA"}],"total_grants":4,"fwci":5.2422,"citation_percentile":0.96116505,"influential_citations":25,"citation_trend":[{"year":2012,"count":15},{"year":2013,"count":13},{"year":2014,"count":7},{"year":2015,"count":13},{"year":2016,"count":8},{"year":2017,"count":12},{"year":2018,"count":8},{"year":2019,"count":13},{"year":2020,"count":20},{"year":2021,"count":12},{"year":2022,"count":12},{"year":2023,"count":17},{"year":2024,"count":11},{"year":2025,"count":3},{"year":2026,"count":3}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/167/1/135/1316662/jcb1671135.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/167/1/135/1316662/jcb1671135.pdf","host_type":"BRONZE"},{"url":"https://rupress.org/jcb/article-pdf/167/1/135/1316662/jcb1671135.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/167/1/135/1531354/jcb1671135.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.200406024","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15479740","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2172516","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2172516","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2172516?pdf=render","host_type":"Europe_PMC"},{"url":"https://dx.doi.org/10.17615/f5r4-1y75","host_type":""},{"url":"http://dx.doi.org/10.1083/jcb.200406024","host_type":""},{"url":"https://dx.doi.org/10.1083/jcb.200406024","host_type":""}],"fields_of_study":["Hippo pathway signaling and YAP/TAZ","Wnt/β-catenin signaling in development and cancer","Cellular Mechanics and Interactions","Medicine","Biology","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Actins","Animals","Cell Differentiation","Crosses, Genetic","Cytoskeleton","DNA Mutational Analysis","Drosophila","Epithelial Cells","Epithelium","Female","Image Processing, Computer-Assisted","Male","Microscopy, Fluorescence","Models, Biological","Mutation","Neurons","Phenotype","Time Factors","Cadherins","Protein Structure, Tertiary","Body Patterning","Adherens Junctions","Drosophila Proteins","Intracellular Signaling Peptides and Proteins"],"keywords":["Adherens junction","Biology","Cell polarity","Cell biology","Epithelial polarity","Polarity (international relations)","Apical cell","Gastrulation","Apical membrane","Apical constriction","Cadherin","Epithelium","Cell","Morphogenesis","Embryo","Genetics","Embryogenesis","Male","DNA Mutational Analysis","Intracellular Signaling Peptides and Proteins","Cell Differentiation","Epithelial Cells","Adherens Junctions","Cadherins","Models, Biological","Actins","Microscopy, Fluorescence","Mutation","Image Processing, Computer-Assisted","Animals","Drosophila Proteins","Drosophila","Female","Research Articles","Crosses, Genetic","Cytoskeleton","Body Patterning"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T03:37:35.240598Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}