{"doi":"10.1083/jcb.200105084","title":"RNA-mediated interaction of Cajal bodies and U2 snRNA genes","abstract":"<jats:p>Cajal bodies (CBs) are nuclear structures involved in RNA metabolism that accumulate high concentrations of small nuclear ribonucleoproteins (snRNPs). Notably, CBs preferentially associate with specific genomic loci in interphase human cells, including several snRNA and histone gene clusters. To uncover functional elements involved in the interaction of genes and CBs, we analyzed the expression and subcellular localization of stably transfected artificial arrays of U2 snRNA genes. Although promoter substitution arrays colocalized with CBs, constructs containing intragenic deletions did not. Additional experiments identified factors within CBs that are important for association with the native U2 genes. Inhibition of nuclear export or targeted degradation of U2 snRNPs caused a marked decrease in the levels of U2 snRNA in CBs and strongly disrupted the interaction with U2 genes. Together, the results illustrate a specific requirement for both the snRNA transcripts as well as the presence of snRNPs (or snRNP proteins) within CBs. Our data thus provide significant insight into the mechanism of CB interaction with snRNA loci, strengthening the putative role for this nuclear suborganelle in snRNP biogenesis.</jats:p>","journal":"The Journal of Cell Biology","year":2001,"id":103945,"datarank":4.954283175553345,"base_score":4.6443908991413725,"endowment":4.6443908991413725,"self_citation_contribution":0.6966586348712059,"citation_network_contribution":4.25762454068214,"self_endowment_contribution":0.6966586348712059,"citer_contribution":4.25762454068214,"corpus_percentile":null,"corpus_rank":null,"citation_count":103,"citer_count":96,"citers_with_citation_signal":89,"citers_with_endowment":89,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":347609,"name":"A. 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Additional experiments identified factors within CBs that are important for association with the native U2 genes. Inhibition of nuclear export or targeted degradation of U2 snRNPs caused a marked decrease in the levels of U2 snRNA in CBs and strongly disrupted the interaction with U2 genes. Together, the results illustrate a specific requirement for both the snRNA transcripts as well as the presence of snRNPs (or snRNP proteins) within CBs. Our data thus provide significant insight into the mechanism of CB interaction with snRNA loci, strengthening the putative role for this nuclear suborganelle in snRNP biogenesis.</jats:p>","is_dataset_classified":null,"base_score":4.6443908991413725,"endowment":4.6443908991413725,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11489914","pmcid":"PMC2196410","openalex_id":"https://openalex.org/W2069332834","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01-GM53034","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM053034","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32-GM08056","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM008056","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM053034-18","title":"Biogenesis of Small Ribonucleoproteins"},{"funder_name":"National Institutes of Health","grant_id":"5T32GM008056-33","title":"Graduate Training in Cell and Molecular Biology"}],"total_grants":6,"fwci":1.3911,"citation_percentile":0.80566951,"influential_citations":8,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":3},{"year":2014,"count":3},{"year":2015,"count":2},{"year":2016,"count":8},{"year":2017,"count":5},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":2},{"year":2022,"count":4},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/154/3/499/1299635/jcb1543499.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/154/3/499/1299635/jcb1543499.pdf","host_type":"BRONZE"},{"url":"https://rupress.org/jcb/article-pdf/154/3/499/1299635/jcb1543499.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/154/3/499/1512786/jcb1543499.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.200105084","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11489914","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2196410","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2196410","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2196410?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1083/jcb.200105084","host_type":""},{"url":"https://dx.doi.org/10.1083/jcb.200105084","host_type":""}],"fields_of_study":["RNA Research and Splicing","Neurogenetic and Muscular Disorders Research","RNA modifications and cancer","Biology","Medicine","0301 basic medicine","0303 health sciences","03 medical and health sciences","Active Transport, Cell Nucleus","Base Sequence","Coiled Bodies","Cyclic AMP Response Element-Binding Protein","Gene Deletion","HeLa Cells","Humans","In Situ Hybridization, Fluorescence","Molecular Sequence Data","Nerve Tissue Proteins","Nuclear Proteins","Nucleic Acid Conformation","Oligonucleotide Array Sequence Analysis","RNA, Small Nuclear","RNA-Binding Proteins","SMN Complex Proteins","Transcription, Genetic"],"mesh_terms":["Base Sequence","HeLa Cells","Humans","Molecular Sequence Data","Nerve Tissue Proteins","Nuclear Proteins","Nucleic Acid Conformation","RNA, Small Nuclear","Transcription, Genetic","RNA-Binding Proteins","Gene Deletion","Cyclic AMP Response Element-Binding Protein","In Situ Hybridization, Fluorescence","Oligonucleotide Array Sequence Analysis","Coiled Bodies","Active Transport, Cell Nucleus","SMN Complex Proteins","Hela Cells"],"keywords":["snRNP","Small nuclear RNA","Biology","Cajal body","Prp24","Small nuclear ribonucleoprotein","Ribonucleoprotein","Gene","Cell biology","Genetics","RNA","RNA splicing","Non-coding RNA","Base Sequence","Transcription, Genetic","Molecular Sequence Data","Active Transport, Cell Nucleus","Nuclear Proteins","RNA-Binding Proteins","Coiled Bodies","Nerve Tissue Proteins","SMN Complex Proteins","Article","RNA, Small Nuclear","Humans","Nucleic Acid Conformation","Cyclic AMP Response Element-Binding Protein","Gene Deletion","In Situ Hybridization, Fluorescence","HeLa Cells","Oligonucleotide Array Sequence Analysis"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-18T22:43:10.856338Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}