{"doi":"10.1083/jcb.147.6.1153","title":"Duplication and Maintenance of Heterochromatin Domains","abstract":"<jats:p>To investigate the mechanisms that assure the maintenance of heterochromatin regions, we took advantage of the fact that clusters of heterochromatin DNA replicate late in S phase and are processed in discrete foci with a characteristic nuclear distribution. At the light microscopy level, within these entities, we followed DNA synthesis, histone H4 acetylation, heterochromatin protein 1 (Hp1α and -β), and chromatin assembly factor 1 (CAF-1). During replication, Hp1α and -β domains of concentration are stably maintained, whereas heterochromatin regions are enriched in both CAF-1 and replication-specific acetylated isoforms of histone H4 (H4Ac 5 and 12). We defined a time window of 20 min for the maintenance of this state. Furthermore, treatment with Trichostatin A (TSA), during and after replication, sustains the H4Ac 5 and 12 state in heterochromatin excluding H4Ac 8 and 16. In comparison, early replication foci, at the same level, did not display any specific enrichment in H4Ac 5 and 12. These data emphasize the specific importance for heterochromatin of the replication-associated H4 isoforms. We propose that perpetuation of heterochromatin involves self-maintenance factors, including local concentration of Hp1α and -β, and that a degree of plasticity is provided by the cycle of H4 acetylation/deacetylation assisted by CAF-1.</jats:p>","journal":"The Journal of Cell Biology","year":1999,"id":634965,"datarank":0.7947476049822055,"base_score":5.298317366548036,"endowment":5.298317366548036,"self_citation_contribution":0.7947476049822055,"citation_network_contribution":0.0,"self_endowment_contribution":0.7947476049822055,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":199,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1647097,"name":"Danièle Roche","orcid":null,"position":1,"is_corresponding":false},{"id":1618077,"name":"Jean-Baptiste Sibarita","orcid":null,"position":2,"is_corresponding":false},{"id":114236,"name":"Bryan M. 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During replication, Hp1α and -β domains of concentration are stably maintained, whereas heterochromatin regions are enriched in both CAF-1 and replication-specific acetylated isoforms of histone H4 (H4Ac 5 and 12). We defined a time window of 20 min for the maintenance of this state. Furthermore, treatment with Trichostatin A (TSA), during and after replication, sustains the H4Ac 5 and 12 state in heterochromatin excluding H4Ac 8 and 16. In comparison, early replication foci, at the same level, did not display any specific enrichment in H4Ac 5 and 12. These data emphasize the specific importance for heterochromatin of the replication-associated H4 isoforms. We propose that perpetuation of heterochromatin involves self-maintenance factors, including local concentration of Hp1α and -β, and that a degree of plasticity is provided by the cycle of H4 acetylation/deacetylation assisted by CAF-1.</jats:p>","is_dataset_classified":null,"base_score":5.298317366548036,"endowment":5.298317366548036,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10601331","pmcid":"PMC2168099","openalex_id":"https://openalex.org/W2146295682","authors":[],"funders":[],"total_grants":0,"fwci":4.5339,"citation_percentile":0.95492318,"influential_citations":0,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":5},{"year":2014,"count":5},{"year":2015,"count":4},{"year":2016,"count":9},{"year":2017,"count":6},{"year":2018,"count":3},{"year":2019,"count":4},{"year":2020,"count":3},{"year":2021,"count":4},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/147/6/1153/1288356/9906120.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/147/6/1153/1288356/9906120.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/147/6/1153/1854618/9906120.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.147.6.1153","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10601331","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.273.5229","host_type":""},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2168099","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2168099","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2168099?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Genomics and Chromatin Dynamics","DNA Repair Mechanisms","Epigenetics and DNA Methylation","Acetylation","Animals","Cell Line","Chromatin Assembly Factor-1","Chromobox Protein Homolog 5","Chromosomal Proteins, Non-Histone","DNA","DNA Replication","DNA-Binding Proteins","Fluorescent Antibody Technique","HeLa Cells","Heterochromatin","Histone Deacetylase Inhibitors","Histone Deacetylases","Histones","Humans","Hydroxamic Acids","Mice","Protein Isoforms","S Phase","Time Factors"],"mesh_terms":["Chromobox Protein Homolog 5","Acetylation","Animals","Cell Line","Chromosomal Proteins, Non-Histone","DNA","DNA Replication","DNA-Binding Proteins","Fluorescent Antibody Technique","HeLa Cells","Heterochromatin","Histone Deacetylases","Histones","Humans","Hydroxamic Acids","Time Factors","S Phase","Protein Isoforms","Mice","Chromatin Assembly Factor-1","Histone Deacetylase Inhibitors","Hela Cells"],"keywords":["Biology","Heterochromatin","Gene duplication","Computational biology","Genetics","Heterochromatin protein 1","Evolutionary biology","Cell biology","Chromosome","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T14:26:32.864314Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}