{"doi":"10.1083/jcb.131.4.875","title":"Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport.","abstract":"<jats:p>COPI and COPII are vesicle coat complexes whose assembly is regulated by the ARF1 and Sar1 GTPases, respectively. We show that COPI and COPII coat complexes are recruited separately and independently to ER (COPII), pre-Golgi (COPI, COPII), and Golgi (COPI) membranes of mammalian cells. To address their individual roles in ER to Golgi transport, we used stage specific in vitro transport assays to synchronize movement of cargo to and from pre-Golgi intermediates, and GDP- and GTP-restricted forms of Sar1 and ARF1 proteins to control coat recruitment. We find that COPII is solely responsible for export from the ER, is lost rapidly following vesicle budding and mediates a vesicular step required for the build-up of pre-Golgi intermediates composed of clusters of vesicles and small tubular elements. COPI is recruited onto pre-Golgi intermediates where it initiates segregation of the anterograde transported protein vesicular stomatitis virus glycoprotein (VSV-G) from the retrograde transported protein p58, a protein which actively recycles between the ER and pre-Golgi intermediates. We propose that sequential coupling between COPII and COPI coats is essential to coordinate and direct bi-directional vesicular traffic between the ER and pre-Golgi intermediates involved in transport of protein to the Golgi complex.</jats:p>","journal":"The Journal of cell 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Aridor","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":6.021023349349527,"endowment":6.021023349349527,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7490291","pmcid":"PMC2200014","openalex_id":"https://openalex.org/W1971476311","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA 58689","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM 42336","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"RRO7273","title":null}],"total_grants":3,"fwci":10.5827,"citation_percentile":0.98872373,"influential_citations":36,"citation_trend":[{"year":2012,"count":10},{"year":2013,"count":9},{"year":2014,"count":9},{"year":2015,"count":7},{"year":2016,"count":8},{"year":2017,"count":4},{"year":2018,"count":11},{"year":2019,"count":4},{"year":2020,"count":8},{"year":2021,"count":11},{"year":2022,"count":5},{"year":2023,"count":11},{"year":2024,"count":7},{"year":2025,"count":8},{"year":2026,"count":4}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/131/4/875/1265335/875.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/131/4/875/1265335/875.pdf","host_type":"BRONZE"},{"url":"https://rupress.org/jcb/article-pdf/131/4/875/1265335/875.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/131/4/875/1479484/875.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.131.4.875","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7490291","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2200014","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2200014","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2200014?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Cellular transport and secretion","Pancreatic function and diabetes","Endoplasmic Reticulum Stress and Disease","Biology","Medicine","ADP-Ribosylation Factor 1","ADP-Ribosylation Factors","Animals","Calcium","Carrier Proteins","Cells, Cultured","Coated Vesicles","Coatomer Protein","Endoplasmic Reticulum","GTP-Binding Proteins","Golgi Apparatus","Intracellular Membranes","Kidney","Membrane Proteins","Microsomes","Monomeric GTP-Binding Proteins","Plasmids","Rats","Receptors, Cytoplasmic and Nuclear","Saccharomyces cerevisiae Proteins","Temperature","Vesicular Transport Proteins","Vesicular stomatitis Indiana virus","Viral Proteins","Lamin B Receptor"],"mesh_terms":["Lamin B Receptor","Animals","Calcium","Carrier Proteins","Cells, Cultured","Endoplasmic Reticulum","Golgi Apparatus","Intracellular Membranes","Kidney","Membrane Proteins","Microsomes","Plasmids","Temperature","Vesicular stomatitis Indiana virus","Viral Proteins","Receptors, Cytoplasmic and Nuclear","Coated Vesicles","GTP-Binding Proteins","Monomeric GTP-Binding Proteins","ADP-Ribosylation Factors","Coatomer Protein","ADP-Ribosylation Factor 1","Saccharomyces cerevisiae Proteins","Vesicular Transport Proteins","Rats"],"keywords":["COPII","COPI","Golgi apparatus","Endoplasmic reticulum","Cell biology","Vesicle","Biology","Vesicular transport protein","GTPase","Vesicular stomatitis virus","Secretory pathway","Biochemistry","Membrane","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Decent work and economic growth"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T06:38:19.967158Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}