{"doi":"10.1083/jcb.123.1.89","title":"Molecules internalized by clathrin-independent endocytosis are delivered to endosomes containing transferrin receptors.","abstract":"<jats:p>We have previously demonstrated that the preendosomal compartment in addition to clathrin-coated vesicles, comprises distinct nonclathrin coated endocytic vesicles mediating clathrin-independent endocytosis (Hansen, S. H., K. Sandvig, and B. van Deurs. 1991. J. Cell Biol. 113:731-741). Using K+ depletion in HEp-2 cells to block clathrin-dependent but not clathrin-independent endocytosis, we have now traced the intracellular routing of these nonclathrin coated vesicles to see whether molecules internalized by clathrin-independent endocytosis are delivered to a unique compartment or whether they reach the same early and late endosomes as encountered by molecules internalized with high efficiency through clathrin-coated pits and vesicles. We find that Con A-gold internalized by clathrin-independent endocytosis is delivered to endosomes containing transferrin receptors. After incubation of K(+)-depleted cells with Con A-gold for 15 min, approximately 75% of Con A-gold in endosomes is colocalized with transferrin receptors. Endosomes containing only Con A-gold may be accounted for either by depletion of existing endosomes for transferrin receptors or by de novo generation of endosomes. Cationized gold and BSA-gold internalized in K(+)-depleted cells are also delivered to endosomes containing transferrin receptors. h-lamp-1-enriched compartments are only reached occasionally within 30 min in K(+)-depleted as well as in control cells. Thus, preendosomal vesicles generated by clathrin-independent endocytosis do not fuse to any marked degree with late endocytic compartments. These data show that in HEp-2 cells, molecules endocytosed without clathrin are delivered to the same endosomes as reached by transferrin receptors internalized through clathrin-coated pits.</jats:p>","journal":"The Journal of cell biology","year":1993,"id":18026,"datarank":6.358797147180355,"base_score":4.68213122712422,"endowment":4.68213122712422,"self_citation_contribution":0.7023196840686331,"citation_network_contribution":5.656477463111722,"self_endowment_contribution":0.7023196840686331,"citer_contribution":5.656477463111722,"corpus_percentile":null,"corpus_rank":null,"citation_count":107,"citer_count":97,"citers_with_citation_signal":90,"citers_with_endowment":90,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":126787,"name":"K Sandvig","orcid":null,"position":1,"is_corresponding":false},{"id":126788,"name":"B van Deurs","orcid":null,"position":2,"is_corresponding":false},{"id":126786,"name":"S H Hansen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.68213122712422,"endowment":4.68213122712422,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8408209","pmcid":"PMC2119820","openalex_id":"https://openalex.org/W2164907841","authors":[],"funders":[],"total_grants":0,"fwci":4.2051,"citation_percentile":0.94586483,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2014,"count":1},{"year":2017,"count":3},{"year":2018,"count":3},{"year":2019,"count":1},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/123/1/89/1260651/89.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/123/1/89/1260651/89.pdf","host_type":"BRONZE"},{"url":"https://rupress.org/jcb/article-pdf/123/1/89/1260651/89.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/123/1/89/1474619/89.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.123.1.89","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8408209","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2119820","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2119820","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2119820?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Cellular transport and secretion","Caveolin-1 and cellular processes","Glycosylation and Glycoproteins Research","Medicine","Biology","Antigens, CD","Artifacts","Biological Transport","Cell Compartmentation","Cell Line","Clathrin","Coated Pits, Cell-Membrane","Concanavalin A","Endocytosis","Histocytochemistry","Humans","Intracellular Membranes","Lysosomal Membrane Proteins","Membrane Glycoproteins","Potassium Deficiency","Receptors, Transferrin"],"mesh_terms":["Biological Transport","Cell Compartmentation","Cell Line","Clathrin","Coated Pits, Cell-Membrane","Concanavalin A","Endocytosis","Histocytochemistry","Humans","Intracellular Membranes","Membrane Glycoproteins","Potassium Deficiency","Receptors, Transferrin","Antigens, CD","Artifacts","Lysosomal Membrane Proteins"],"keywords":["Endosome","Endocytosis","Clathrin","Endocytic cycle","Transferrin receptor","Cell biology","Receptor-mediated endocytosis","Vesicle","Transferrin","Biology","Bulk endocytosis","Pinocytosis","Receptor","Intracellular","Biochemistry","Membrane"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-03T04:35:04.532373Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}