{"doi":"10.1083/jcb.109.3.1219","title":"Simultaneous measurements of cytosolic calcium and secretion in single bovine adrenal chromaffin cells by fluorescent imaging of fura-2 in cocultured cells.","abstract":"<jats:p>The cytosolic free calcium concentration ([Ca2+]i) and exocytosis of chromaffin granules were measured simultaneously from single, intact bovine adrenal chromaffin cells using a novel technique involving fluorescent imaging of cocultured cells. Chromaffin cell [Ca2+]i was monitored with fura-2. To simultaneously follow catecholamine secretion, the cells were cocultured with fura-2-loaded NIH-3T3t cells, a cell line chosen because of their irresponsiveness to chromaffin cell secretagogues but their large Ca2+ response to ATP, which is coreleased with catecholamine from the chromaffin cells. In response to the depolarizing stimulus nicotine (a potent secretagogue), chromaffin cell [Ca2+]i increased rapidly. At the peak of the response, [Ca2+]i was evenly distributed throughout the cell. This elevation in [Ca2+]i was followed by a secretory response which originated from the entire surface of the cell. In response to the inositol 1,4,5-trisphosphate (InsP3)-mobilizing agonist angiotensin II (a weak secretagogue), three different responses were observed. Approximately 30% of chromaffin cells showed no rise in [Ca2+]i and did not secrete. About 45% of the cells responded with a large (greater than 200 nM), transient elevation in [Ca2+]i and no detectable secretory response. The rise in [Ca2+]i was nonuniform, such that peak [Ca2+]i was often recorded only in one pole of the cell. And finally, approximately 25% of cells responded with a similar Ca2+-transient to that described above, but also gave a secretory response. In these cases secretion was polarized, being confined to the pole of the cell in which the rise in [Ca2+]i was greatest.(ABSTRACT TRUNCATED AT 250 WORDS)</jats:p>","journal":"The Journal of cell biology","year":1989,"id":24636,"datarank":8.842753318622588,"base_score":4.836281906951478,"endowment":4.836281906951478,"self_citation_contribution":0.7254422860427218,"citation_network_contribution":8.117311032579867,"self_endowment_contribution":0.7254422860427218,"citer_contribution":8.117311032579867,"corpus_percentile":null,"corpus_rank":null,"citation_count":125,"citer_count":111,"citers_with_citation_signal":108,"citers_with_endowment":108,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":147265,"name":"T R Jackson","orcid":null,"position":1,"is_corresponding":false},{"id":147266,"name":"A J O'Sullivan","orcid":null,"position":2,"is_corresponding":false},{"id":147267,"name":"R B Moreton","orcid":null,"position":3,"is_corresponding":false},{"id":147268,"name":"M J Berridge","orcid":null,"position":4,"is_corresponding":false},{"id":147269,"name":"R D Burgoyne","orcid":null,"position":5,"is_corresponding":false},{"id":147264,"name":"T R Cheek","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.836281906951478,"endowment":4.836281906951478,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2768340","pmcid":"PMC2115761","openalex_id":"https://openalex.org/W2109440146","authors":[],"funders":[],"total_grants":0,"fwci":13.6902,"citation_percentile":0.99488578,"influential_citations":3,"citation_trend":[{"year":2012,"count":1},{"year":2019,"count":1},{"year":2021,"count":1},{"year":2023,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/109/3/1219/1058514/1219.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/109/3/1219/1058514/1219.pdf","host_type":"BRONZE"},{"url":"https://rupress.org/jcb/article-pdf/109/3/1219/1058514/1219.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/109/3/1219/1466710/1219.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.109.3.1219","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2768340","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2115761","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2115761","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2115761?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Antimicrobial Peptides and Activities","Aquaculture disease management and microbiota","RNA Interference and Gene Delivery","Biology","Medicine","Adenosine Triphosphate","Adrenal Medulla","Angiotensin II","Animals","Benzofurans","Calcium","Cell Communication","Cells, Cultured","Chromaffin Granules","Chromaffin System","Cytosol","Exocytosis","Fluorescent Dyes","Fura-2","Mice","Nicotine"],"mesh_terms":["Adenosine Triphosphate","Adrenal Medulla","Angiotensin II","Animals","Benzofurans","Calcium","Cell Communication","Cells, Cultured","Chromaffin Granules","Chromaffin System","Cytosol","Exocytosis","Fluorescent Dyes","Nicotine","Fura-2","Mice"],"keywords":["Secretagogue","Chromaffin cell","Fura-2","Exocytosis","Secretion","Biology","Adrenal medulla","Endocrinology","Internal medicine","Catecholamine","Cell culture","Calcium","Depolarization","Cytosol","Biophysics","Cell biology","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T22:53:39.209821Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}