{"doi":"10.1083/jcb.105.1.163","title":"Purification and characterization of constitutive secretory vesicles from yeast.","abstract":"<jats:p>We have developed a purification procedure for the isolation of constitutive post-Golgi secretory vesicles from Saccharomyces cerevisiae. Although the post-Golgi stage of the secretion pathway is normally very rapid, we have used a temperature-sensitive secretory mutant, sec 6-4, to greatly expand the population of secretory vesicles. Following invertase as a marker, intact vesicles are enriched 36-fold from the crude lysate. The final preparation contains few contaminants as assessed by morphologic and biochemical examination. Three proteins (110, 40-45, and 18 kD) co-purify with the vesicle marker enzyme invertase. Metabolic labeling experiments indicate that these vesicle-associated proteins are synthesized during the period of vesicle accumulation. They are not apparent in the corresponding fractions from wild-type cells. Analysis of these proteins indicates that the 110-kD protein is a major glycoprotein residing in the vesicle lumen, while the 40-45- and 18-kD proteins are not glycosylated and are firmly associated with the vesicle membrane, each with at least one domain exposed on the cytoplasmic surface.</jats:p>","journal":"The Journal of cell biology","year":1987,"id":36420,"datarank":11.976474134491799,"base_score":5.19295685089021,"endowment":5.19295685089021,"self_citation_contribution":0.7789435276335317,"citation_network_contribution":11.197530606858267,"self_endowment_contribution":0.7789435276335317,"citer_contribution":11.197530606858267,"corpus_percentile":null,"corpus_rank":null,"citation_count":179,"citer_count":164,"citers_with_citation_signal":151,"citers_with_endowment":151,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":42.5,"fair_percentile":4.5988586774085265,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":184446,"name":"P J Novick","orcid":null,"position":1,"is_corresponding":false},{"id":184445,"name":"N C Walworth","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.19295685089021,"endowment":5.19295685089021,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"3301865","pmcid":"PMC2114885","openalex_id":"https://openalex.org/W2065852288","authors":[],"funders":[{"funder_name":"NCRR NIH HHS","grant_id":"BRSG RR05358","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM35370","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM07223","title":null}],"total_grants":3,"fwci":3.461,"citation_percentile":0.93999446,"influential_citations":13,"citation_trend":[{"year":2012,"count":2},{"year":2014,"count":2},{"year":2015,"count":4},{"year":2016,"count":1},{"year":2017,"count":3},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://rupress.org/jcb/article-pdf/105/1/163/1055294/163.pdf","host_type":"journal"},{"url":"https://rupress.org/jcb/article-pdf/105/1/163/1055294/163.pdf","host_type":"BRONZE"},{"url":"https://rupress.org/jcb/article-pdf/105/1/163/1055294/163.pdf","host_type":"publisher"},{"url":"https://rupress.org/jcb/article-pdf/105/1/163/1465361/163.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1083/jcb.105.1.163","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/3301865","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2114885","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2114885","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2114885?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Cellular transport and secretion","Biotin and Related Studies","Photosynthetic Processes and Mechanisms","Biology","Medicine","Cell Fractionation","Fungal Proteins","Intracellular Membranes","Protein Processing, Post-Translational","Saccharomyces cerevisiae"],"mesh_terms":["Cell Fractionation","Fungal Proteins","Intracellular Membranes","Protein Processing, Post-Translational","Saccharomyces cerevisiae"],"keywords":["Vesicle","Biology","Secretory Vesicle","Golgi apparatus","Secretory protein","Secretory pathway","Secretion","Biochemistry","Glycoprotein","Cytoplasm","Population","Invertase","Cell biology","Enzyme","Endoplasmic reticulum","Membrane"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T15:56:13.870964Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":100.0,"fair_a":70.0,"fair_i":0.0,"fair_r":0.0,"fair_zscore":-0.6138,"fair_rationale":{"fair_score":42.5,"has_llm":false,"dimensions":{"F":{"name":"Findable","score":100.0,"criteria":[{"key":"f_has_doi","label":"Has a persistent DOI","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"DOI present","rationale":null},{"key":"f_repository_presence","label":"Indexed in repositories / literature DBs","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"datacite=0, pmcid=True, pmid=True","rationale":null},{"key":"f_persistent_ids","label":"Resolvable scholarly identifiers (OpenAlex)","kind":"deterministic","weight":0.5,"fraction":1.0,"signal":"OpenAlex id present","rationale":null}]},"A":{"name":"Accessible","score":70.0,"criteria":[{"key":"a_open_access","label":"Open Access / files deposited","kind":"deterministic","weight":1.5,"fraction":0.5,"signal":"files/OA location present but not flagged OA","rationale":null},{"key":"a_retrievable","label":"Free full text retrievable","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"9 OA location(s)","rationale":null}]},"I":{"name":"Interoperable","score":0.0,"criteria":[{"key":"i_linked_data","label":"Linked datasets / DataCite relations","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"linked_datasets=0, datacite=0","rationale":null},{"key":"i_standard_ids","label":"References data via standard accessions","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"accessions=0, trials=0","rationale":null}]},"R":{"name":"Reusable","score":0.0,"criteria":[{"key":"r_license","label":"Clear, open reuse license","kind":"deterministic","weight":1.5,"fraction":0.0,"signal":"no license","rationale":null},{"key":"r_downloads","label":"Demonstrated reuse (downloads)","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"downloads=0","rationale":null},{"key":"r_version","label":"Versioned / maintained","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"no version chain","rationale":null},{"key":"r_dataset","label":"Classified as a data resource","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"not a dataset","rationale":null}]}},"suggestions":["Attach a clear, open reuse license (e.g. CC-BY or CC0).","Link the underlying datasets via DOIs / DataCite relations.","Reference data using standard accessions (e.g. GEO, PDB, ClinicalTrials.gov).","Maintain explicit versioning for the dataset.","Make the paper/data Open Access or deposit the files in an open repository."],"model":null,"agent_version":"fair_agent_v1","fulltext_source":"epmc_xml"},"fair_model":null,"fair_agent_version":"fair_agent_v1","fair_fulltext_source":"epmc_xml","fair_has_llm":false,"fair_computed_at":"2026-06-10T16:14:21.118908Z","clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}