{"doi":"10.1080/07391102.2012.706073","title":"Affinity maturation of antiHER2 monoclonal antibody MIL5 using an epitope-specific synthetic phage library by computational design","abstract":null,"journal":"Journal of Biomolecular Structure and Dynamics","year":2013,"id":621069,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1603554,"name":"Ming Lv","orcid":null,"position":1,"is_corresponding":false},{"id":1270305,"name":"Xinying Li","orcid":"0009-0008-9783-1914","position":2,"is_corresponding":false},{"id":328689,"name":"Jing Geng","orcid":"0000-0003-3432-1008","position":3,"is_corresponding":false},{"id":418221,"name":"Yan Li","orcid":"0000-0002-7884-8074","position":4,"is_corresponding":false},{"id":1603556,"name":"Jiyan Zhang","orcid":null,"position":5,"is_corresponding":false},{"id":851542,"name":"Zhou Lin","orcid":"0000-0003-3271-5345","position":6,"is_corresponding":false},{"id":1603557,"name":"Jiannan Feng","orcid":null,"position":7,"is_corresponding":false},{"id":1603559,"name":"Beifen Shen","orcid":null,"position":8,"is_corresponding":false},{"id":1603553,"name":"Chunxia Qiao","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Affinity maturation of antiHER2 monoclonal antibody MIL5 using an epitope-specific synthetic phage library by computational design","abstract":"Increased affinities mainly equal to improved biological efficacy in many cases. By now, display methods including phage library are widely exploited to obtain higher affinity antibodies. Traditional library methods mainly focus on complementary determining region mutagenesis, in which extensive screening of variant combinations as well as large library capacity is required to find higher affinity clones. In this study, based on the modeling 3D complex structure of antigen (HER2)-antibody (MIL5) complex, the key residues of contact surface were predicted and identified to guide the synthetic phage library design. Then, epitope-specific site-directed mutagenesis phage library comprised of MIL5_scFv mutants was constructed, from which a higher affinity single chain antibody (M5scFv_ph) was screened out. Following experimental results showed that the novel antibody M5scFv_ph retained superimposed epitope to the parent antibody MIL5_scFv, and possessed similar tumor growth inhibitory activity in vivo on ovarian carcinoma xenografts.","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23003339","pmcid":null,"openalex_id":"https://openalex.org/W2175294133","authors":[],"funders":[],"total_grants":0,"fwci":0.4477,"citation_percentile":0.6871828,"influential_citations":0,"citation_trend":[{"year":2014,"count":2},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://www.tandfonline.com/doi/pdf/10.1080/10256018808623883","host_type":"publisher"},{"url":"https://doi.org/10.1080/07391102.2012.706073","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23003339","host_type":"repository"}],"fields_of_study":["Monoclonal and Polyclonal Antibodies Research","Glycosylation and Glycoproteins Research","Protein purification and stability"],"mesh_terms":["Animals","Antibodies, Monoclonal","Epitopes","Antineoplastic Agents","Carcinoma","Computer Simulation","Female","Humans","Mice, Nude","Models, Molecular","Ovarian Neoplasms","Protein Binding","Drug Design","Protein Structure, Secondary","Epitope Mapping","Erb-b2 Receptor Tyrosine Kinases","Receptor, ErbB-2","Peptide Library","Amino Acid Motifs","Xenograft Model Antitumor Assays","Cell Line, Tumor","Tumor Burden","Mice","Protein Interaction Domains and Motifs","HEK293 Cells"],"keywords":["Epitope","Phage display","Mutagenesis","Monoclonal antibody","Affinity maturation","Antibody","Mimotope","Peptide library","Chemistry","Epitope mapping","Molecular biology","Linear epitope","Conformational epitope","Mutant","Phagemid","Biology","Biochemistry","Peptide sequence","Bacteriophage","Escherichia coli","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T13:07:34.742976Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}