{"doi":"10.1074/mcp.o113.033571","title":"A Second-generation Protein–Protein Interaction Network of Helicobacter pylori","abstract":null,"journal":"Molecular & Cellular Proteomics","year":2014,"id":601848,"datarank":0.6261580904843456,"base_score":4.174387269895637,"endowment":4.174387269895637,"self_citation_contribution":0.6261580904843456,"citation_network_contribution":0.0,"self_endowment_contribution":0.6261580904843456,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":64,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1543288,"name":"Arnaud Ceol","orcid":null,"position":1,"is_corresponding":false},{"id":388234,"name":"Seesandra V. Rajagopala","orcid":"0000-0002-3160-8882","position":2,"is_corresponding":false},{"id":1543289,"name":"Roberto Mosca","orcid":null,"position":3,"is_corresponding":false},{"id":1543290,"name":"Gabriella Siszler","orcid":null,"position":4,"is_corresponding":false},{"id":1543291,"name":"Nadja Wermke","orcid":null,"position":5,"is_corresponding":false},{"id":490406,"name":"Patricia Sikorski","orcid":"0000-0003-4609-1174","position":6,"is_corresponding":false},{"id":1543294,"name":"Frank Schwarz","orcid":null,"position":7,"is_corresponding":false},{"id":1543295,"name":"Matthias Schick","orcid":null,"position":8,"is_corresponding":false},{"id":21507,"name":"Stefan Wuchty","orcid":"0000-0001-8916-6522","position":9,"is_corresponding":false},{"id":307113,"name":"Patrick Aloy","orcid":"0000-0002-3557-0236","position":10,"is_corresponding":false},{"id":738239,"name":"Peter Uetz","orcid":"0000-0001-6194-4927","position":11,"is_corresponding":false},{"id":1543287,"name":"Roman Häuser","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Second-generation Protein–Protein Interaction Network of Helicobacter pylori","abstract":"Helicobacter pylori infections cause gastric ulcers and play a major role in the development of gastric cancer. In 2001, the first protein interactome was published for this species, revealing over 1500 binary protein interactions resulting from 261 yeast two-hybrid screens. Here we roughly double the number of previously published interactions using an ORFeome-based, proteome-wide yeast two-hybrid screening strategy. We identified a total of 1515 protein-protein interactions, of which 1461 are new. The integration of all the interactions reported in H. pylori results in 3004 unique interactions that connect about 70% of its proteome. Excluding interactions of promiscuous proteins we derived from our new data a core network consisting of 908 interactions. We compared our data set to several other bacterial interactomes and experimentally benchmarked the conservation of interactions using 365 protein pairs (interologs) of E. coli of which one third turned out to be conserved in both species.","is_dataset_classified":null,"base_score":4.174387269895637,"endowment":4.174387269895637,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24627523","pmcid":"PMC4014287","openalex_id":"https://openalex.org/W2162117109","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"RO1GM79710","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM079710","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01GM79710","title":null}],"total_grants":3,"fwci":6.712,"citation_percentile":0.97395992,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2015,"count":5},{"year":2016,"count":12},{"year":2017,"count":6},{"year":2018,"count":7},{"year":2019,"count":7},{"year":2020,"count":5},{"year":2021,"count":8},{"year":2022,"count":2},{"year":2023,"count":3},{"year":2024,"count":4},{"year":2025,"count":1},{"year":2026,"count":3}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://www.mcponline.org/content/mcprot/13/5/1318.full.pdf","host_type":"journal"},{"url":"https://www.mcponline.org/content/mcprot/13/5/1318.full.pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1535947620331054?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1535947620331054?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/mcp.O113.033571","host_type":"publisher"},{"url":"https://doi.org/10.1074/mcp.o113.033571","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24627523","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4014287","host_type":"repository"}],"fields_of_study":["Helicobacter pylori-related gastroenterology studies","Microbial Metabolites in Food Biotechnology","Genomics and Phylogenetic Studies"],"mesh_terms":["Amino Acid Sequence","Bacterial Proteins","Open Reading Frames","Helicobacter pylori","Conserved Sequence","Proteome","Two-Hybrid System Techniques","Protein Interaction Mapping","Proteomics","Protein Interaction Maps"],"keywords":["Interactome","Proteome","Protein–protein interaction","Two-hybrid screening","Interaction network","Computational biology","Helicobacter pylori","Biology","Yeast","Genetics","Protein Interaction Networks","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T17:37:30.974279Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}