{"doi":"10.1074/mcp.m115.053249","title":"Quantitative Proteomics Reveals β2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability","abstract":null,"journal":"Molecular & Cellular Proteomics","year":2016,"id":630343,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1632931,"name":"Jingi Bae","orcid":null,"position":1,"is_corresponding":false},{"id":572422,"name":"Sehyun Chae","orcid":"0000-0001-6904-0623","position":2,"is_corresponding":false},{"id":1632932,"name":"Jin Hyoung Kim","orcid":null,"position":3,"is_corresponding":false},{"id":1632934,"name":"Jong-Hee Han","orcid":null,"position":4,"is_corresponding":false},{"id":32946,"name":"Daehee Hwang","orcid":"0000-0002-7553-0044","position":5,"is_corresponding":false},{"id":1632935,"name":"Sang-Won Lee","orcid":null,"position":6,"is_corresponding":false},{"id":1334103,"name":"Jeong Hun Kim","orcid":"0000-0002-5637-1121","position":7,"is_corresponding":false},{"id":1334102,"name":"Dong Hyun Jo","orcid":"0000-0002-6320-6829","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Quantitative Proteomics Reveals β2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability","abstract":"Retinal vascular hyperpermeability causes macular edema, leading to visual deterioration in retinal diseases such as diabetic retinopathy and retinal vascular occlusion. Dysregulation of junction integrity between endothelial cells by vascular endothelial growth factor (VEGF) was shown to cause retinal vascular hyperpermeability. Accordingly, anti-VEGF agents have been used to treat retinal vascular hyperpermeability. However, they can confer potential toxicity through their deleterious effects on maintenance and survival of neuronal and endothelial cells in the retina. Thus, it is important to identify novel therapeutic targets for retinal vascular hyperpermeability other than VEGF. Here, we prepared murine retinas showing VEGF-induced vascular leakage from superficial retinal vascular plexus and prevention of VEGF-induced leakage by anti-VEGF antibody treatment. We then performed comprehensive proteome profiling of these samples and identified retinal proteins for which abundances were differentially expressed by VEGF, but such alterations were inhibited by anti-VEGF antibody. Functional enrichment and network analyses of these proteins revealed the β2 integrin pathway, which can prevent dysregulation of junction integrity between endothelial cells through cytoskeletal rearrangement, as a potential therapeutic target for retinal vascular hyperpermeability. Finally, we experimentally demonstrated that inhibition of the β2 integrin pathway salvaged VEGF-induced retinal vascular hyperpermeability, supporting its validity as an alternative therapeutic target to anti-VEGF agents.","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26969716","pmcid":"PMC4858948","openalex_id":"https://openalex.org/W2294524542","authors":[],"funders":[{"funder_name":"National Research Foundation of Korea","grant_id":"2009–0090895, 2012–0009544, 2015M3A9E6028949, 2015M3A9E6028947, 2012M3A9B9036675, 2014M3C7A1046047","title":null},{"funder_name":"Ministry of Education, Science and Technology","grant_id":"IBS-R013-G1–2015-a00","title":null},{"funder_name":"Seoul National University","grant_id":"800–20140542","title":null}],"total_grants":3,"fwci":2.6292,"citation_percentile":0.88683534,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2017,"count":2},{"year":2018,"count":4},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":3},{"year":2023,"count":2},{"year":2025,"count":3}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://www.mcponline.org/content/mcprot/15/5/1681.full.pdf","host_type":"journal"},{"url":"https://www.mcponline.org/content/mcprot/15/5/1681.full.pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1535947620336069?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1535947620336069?httpAccept=text/plain","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1074/mcp.M115.053249","host_type":"publisher"},{"url":"https://doi.org/10.1074/mcp.m115.053249","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26969716","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4858948","host_type":"repository"},{"url":"https://hdl.handle.net/10371/191584","host_type":"repository"}],"fields_of_study":["Retinal Diseases and Treatments","Retinal Development and Disorders","Barrier Structure and Function Studies","Animals","Antibodies","CD18 Antigens","Capillary Permeability","Cytoskeleton","Disease Models, Animal","Gene Expression Regulation","Mice","Protein Interaction Maps","Proteomics","Retinal Diseases","Retinal Vessels","Signal Transduction","Vascular Endothelial Growth Factor A"],"mesh_terms":["Animals","Antibodies","Capillary Permeability","Cytoskeleton","Disease Models, Animal","Gene Expression Regulation","Retinal Diseases","Retinal Vessels","Signal Transduction","CD18 Antigens","Proteomics","Vascular Endothelial Growth Factor A","Mice","Protein Interaction Maps"],"keywords":["Vascular permeability","Vascular endothelial growth factor","Retinal","Vascular endothelial growth factor A","Vascular endothelial growth factor B","Retina","Cell biology","Biology","Cancer research","Pathology","Medicine","Ophthalmology","Neuroscience","VEGF receptors"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pxd"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T21:01:12.750078Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}