{"doi":"10.1074/jbc.ra120.013419","title":"19F NMR reveals the conformational properties of free thrombin and its zymogen precursor prethrombin-2","abstract":"-bound form. The results cast doubts on recent hypotheses that free thrombin is zymogen-like and transitions to protease-like forms upon ligand binding. Rather, they validate the scenario emerged from previous findings of X-ray crystallography and rapid kinetics supporting a pre-existing equilibrium between open (E) and closed (E*) forms of the active site. In this scenario, prethrombin-2 is more dynamic and exists predominantly in the E* form, whereas thrombin is more rigid and exists predominantly in the E form. Ligand binding to thrombin takes place exclusively in the E form without significant changes in the overall conformation. In summary, these results disclose the structural architecture of the free forms of thrombin and prethrombin-2, consistent with an E*-E equilibrium and providing no evidence that free thrombin is zymogen-like.","journal":"Journal of Biological Chemistry","year":2020,"id":83419,"datarank":0.3958585994422889,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.0,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9589,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":429008,"name":"Prafull S. Gandhi","orcid":"0000-0001-7949-4899","position":1,"is_corresponding":false},{"id":105632,"name":"Zhiwei Chen","orcid":"0000-0002-4511-2888","position":2,"is_corresponding":false},{"id":429009,"name":"Sarah K. Koester","orcid":"0000-0003-4626-504X","position":3,"is_corresponding":false},{"id":429634,"name":"Gregory T. DeKoster","orcid":null,"position":4,"is_corresponding":false},{"id":429010,"name":"Carl Frieden","orcid":"0000-0002-4471-2427","position":5,"is_corresponding":false},{"id":369910,"name":"Enrico Di","orcid":"0000-0003-2300-4891","position":6,"is_corresponding":false},{"id":429007,"name":"Eliza A. Ruben","orcid":"0000-0003-2748-5530","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:54:09.383533Z","pmid":"32358061","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}